Effects of Azelaic Acid Adjuvant on Humoral Immune Response Induced by Hepatitis A Virus Attenuated Live Vaccine in Mice

doi:10.3969/j.issn.1674-5817.2018.02.005

Abstract

Abstract: Objective To investigate the effect of azelaic acid as an adjuvant on humoral immune response induced by hepatitis A virus (HAV) attenuated live vaccine (HepA-1) in mice to evaluate its adjuvant effect.Methods The ICR mice were injected respectively with HepA-1(18EU) and Al(OH)₃(300 mg) and four different doses(0.5 mg,1 mg,2 mg,3 mg) of azelaic acid as adjuvant group,300 µL for each and one immunization.At the same time,set up the blank group,pure vaccine group,aluminum adjuvant control group.The sera from tail vein blood of mice were collected at the end of 4,8,12 and 16 weeks after immunization and the specific IgG antibody against HAV were detected by indirect ELISA method.Results In addition to the blank group,mice in various groups generated Anti-HAV IgG at 4,8,12,16 weeks after the immunization.The antibody levels increased as time went on and reached peak at the 8th and 12th week,then decreased gradually.At 4 weeks,the level of anti-hepatitis A virus (HAV) IgG produced by adjuvant group 4 (azelaic acid 3 mg) was significantly higher than aluminum adjuvant control group (P<0.05,t = 2.640).At the same time,the level of anti-hepatitis A virus (HAV) IgG in adjuvant group 4 was significantly higher at 4th week,8th week and 12th week than pure antigen group (P<0.05,t = 3.134,2.751,2.743).Conclusion Appropriate dose of azelaic acid can obviously enhance the HepA-1 induced humoral immune response in mice.Azelaic acid is expected to become a new adjuvant for human vaccines.

Key words: Azelaic acid, Vaccine adjuvant, Hepatitis A virus attenuated live vaccine(HepA-1)

CLC Number:

Q95-33

LI Xiao-hong, JIN Xiao, JIA Sen-quan, HU Ning-zhu, HU Yun-zhan, LI Jian-fang. Effects of Azelaic Acid Adjuvant on Humoral Immune Response Induced by Hepatitis A Virus Attenuated Live Vaccine in Mice[J]. Laboratory Animal and Comparative Medicine, 2018, 38(2): 105-110.

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