Molecular Characteristics and Pathogenicity of Newcastle Disease Virus from Domestic Ducks in China

doi:10.3969/j.issn.1674-5817.2015.01.014

Laboratory Animal and Comparative Medicine ›› 2015, Vol. 35 ›› Issue (1): 65-70.DOI: 10.3969/j.issn.1674-5817.2015.01.014

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Molecular Characteristics and Pathogenicity of Newcastle Disease Virus from Domestic Ducks in China

WU Wei¹, LIU Huai-ran², LIU Sheng-wang^1,2, CHEN Hong-yan^1,2

1. Northeast Agricultural University, Harbin 150030, China;
2. State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin 150001, China

Received:2014-12-08 Online:2015-02-25 Published:2015-02-25

Abstract

Abstract: Objective To study the molecular characteristics of Newcastle disease virus from domestic ducks, and then compare the pathogenicity in SPF chicken and ducks. Methods By cloning and sequencing the complete genomic sequence of Newcastle disease virus (NDV) strain Du/CH/LGD/358/2011, and then conducted the pathogenicity test in SPF chicken and ducks. Results The genome was 15 198 nucleotides (nt) in length, followed the “rule of six” that is required for replication of NDV strains, and encoded six structural polypeptides. The cleavage motif of F protein was 112 ERQER/L117, which was the characteristic of lentogenic strain. Phylogenetic analysis based on complete genomic sequences and partial sequences of F gene revealed that Du/CH/LGD/358/2011 belonged to class I genotype 3b, an independent clade closely related to Hong Kong LBM viruses. In addition, the pathogenic results between SPF chickens and SPF ducks was hardly varied. Conclusion Du/CH/LGD/358/2011 belonged to lentogenic strain, both SPF chicken and ducks could estimating be used fpr the pathogenicity. This study provided the theoretical basis for mornitoring on SPF ducks.

Key words: Newcastle disease virus, SPF duck, SPF chicken, Pathogenicity, Molecular characteristics

CLC Number:

Q95-33

WU Wei, LIU Huai-ran, LIU Sheng-wang, CHEN Hong-yan. Molecular Characteristics and Pathogenicity of Newcastle Disease Virus from Domestic Ducks in China[J]. Laboratory Animal and Comparative Medicine, 2015, 35(1): 65-70.

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Molecular Characteristics and Pathogenicity of Newcastle Disease Virus from Domestic Ducks in China

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