Abstract: Objective To prepare antiserum against VP3 protein and propagate goose parvovirus (GPV) H in duck embryo and duck embryo fibroblasts (DEFs). Methods The prokaryotic expression vector pET30a-VP3 were constructed based on VP3 seqeucne of GPV H. VP3 protein was induced, expressd and purified, then immunized the New Zealand white rabbit. The VP3 antiserum was collected, purified and verified by Western blotting. GPV H fluid was inoculated into shaoxing duck embryo and DEFs, and the propagation of GPV was identified by PCR and indirect immunofuorescence. Result The VP3 antiserum was successfully prepared, and GPV H was well propagated in shaoxing duck embryo and DEFs. Conclusion VP3 antiserum preparation and GPV replication in duck embryo and DEFs may be provided basis for GPV molecular biological characteristic and pathogenic mechanism research.

Key words: Goose parvovirus (GPV), VP3 gene, VP3 antiserum, Propagation