Experimental Study on Treatment of Rheumatoid Arthritis in Rabbits by Injection of Deer Melon Polypeptide

doi:10.3969/j.issn.1674-5817.2016.03.009

Abstract

Abstract: Objective To study the mechanism of the treatment on rheumatoid arthrist (RA) in rabbits by deer melon polypeptide. Methods Calf cartilage II collagen+Freund’s complete adjuvant (0.1 mL) was injected intra-articular in right knee of rabbits to prepare RA model. The model rabbits were randomly divided into model group, low and high dosage deer melon polypeptide group (Group A and B) with 1 mg/d or 2 mg/d. The paw swelling were calculated at 1, 7, 14, 21 days after treatment, the hyaluronic acid (HA), interleukin-1β (IL-1β), matrix metalloproteinase (MMP-3) and bone morphogenetic protein (BMP) content in joint effusion were measured. Results Compared with the model group, paw swelling were occurred in the rabbits of group A and B, MMP-3 and IL-1β were significantly decreased, BMP and HA significantly increased(P<0.05), but no difference between groups A and B (P<0.05). Conclusion The deer melon polypeptide had the treatment effects on RA in rabbit by improving the RA synthesis and BMP secretion in articular, and inhibiting the erosion of MMP-3 and IL-1β on synovial.

Key words: Rabbit, Rheumatoid arthritis (RA), Deer melon polypeptide

CLC Number:

Q95-33

FENG Yan-qin, AO Ji-bo, YANG Min, CHEN Jian-ming, CHEN De-sen. Experimental Study on Treatment of Rheumatoid Arthritis in Rabbits by Injection of Deer Melon Polypeptide[J]. Laboratory Animal and Comparative Medicine, 2016, 36(3): 204-207.

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