Identification of Mouse Prss37 Transcription Initiation Site and Preliminary Analysis of Its Transcription Regulation

doi:10.3969/j.issn.1674-5817.2016.02.003

Abstract

Abstract: Objective To determine the transcription initiation site of mouse Prss37 gene, and verify the tissue specificity of the promoter in vivo using transgenic technology. Methods The transcription initiation site was identified by 5' rapid amplification of cDNA ends (5' RACE ) used to analyze the mRNA of testis from C57BL/6J mice . The plasmids including different lengths of promoter (1kb, 4kb, and 6kb) and luciferase gene were constructed. Then, these plasmids were injected into male pronucleus by microinjection technique to obtain luciferase positive transgenic mice. Results The transcription start site of Prss37 gene was located at the 40bp site upstream of the predicted site reported in GeneBankTM (NM_026317.2). Moreover, three kinds of transgenic mice were successfully established, but the expression of luciferase gene was only detectable in transgenic mice driven by 1kb promoter. The higher expression level of luciferase was found in brain, testis and epididymis tissues. Conclusion The transgenic mice expressing luciferase in testis and epididymis tissues were successfully established, which laid a foundation for further research on the transcriptional regulation of Prss37 gene.

Key words: Prss37 gene, Transcription, Transgenic mice, Luciferase gene

CLC Number:

Q95-33

WANG Jin-jin, KUANG Fang-zhe, ZHUANG Hua, LU Cui-jie, KUANG Ying. Identification of Mouse Prss37 Transcription Initiation Site and Preliminary Analysis of Its Transcription Regulation[J]. Laboratory Animal and Comparative Medicine, 2016, 36(2): 93-100.

References

[1] Ikawa M, Inoue N, Benham AM, et al.Fertilization: A sperm’s journey to and interaction with the oocyte[J]. J Clin Investigat, 2010, 120(4):984-994.
[2] Muro Y, Okabe M.Mechanisms of fertilization-a view from the study of gene-manipulated mice[J]. J Androl, 2011, 32(3):218-225.
[3] Rawlings ND, Barrett AJ.Evolutionary families of peptidases[J]. Biochem, J, 1993, 290(Pt1):205-218.
[4] Kawano N, Kang W, Yamashita M,et al.Mice lacking two sperm serine proteases,ACR and PRSS21,are subfertile,but the mutant sperm are infertile in vitro[J]. Biol Reprod, 2010, 83(3):359-369.
[5] Honda A, Yamagata K, Sugiura S, et al.A mouse serine protease TESP5 is selectively included into lipid rafts of sperm membrane presumably as glycosylphosphatidylinositol-anchored protein[J].J Biol Chem, 2002, 277(19):16976-16984.
[6] Gyamera-Acheampong, C, Tantibhedhyangkul J, Weerachatyanukul W.et al,Sperm from mice genetically deficient for the PCSK4 proteinase exhibit accelerated capacitation,precocious acrosome reaction,reduced binding to egg zona pellucida,and impaired fertilizing ability[J].Biol Reprod, 2006, 74(4):666-673.
[7] Gyamera-Acheampong C, Mbikay M.Proprotein convertase subtilisin/kexin type 4 in mammalian fertility: a review[J].Hum Reprod Update, 2009, 15(2):237-247.
[8] Shen C, Kuang Y, Liu J, et al.Prss37 is required for male fertility in the mouse[J].Biol Reprod, 2013, 88(5):1-11.
[9] Contag PR, Olomu IN, Stevenson DK, et al.Bioluminescent indicators in living mammals[J]. Nat Med, 1998, 4(2):245-247.
[10] Zhang W, Contag PR, Madan A, et al.Bioluminescence for biological sensing in living mammals[J].Adv Exp Med Biol, 1999, 471:775-784.
[11] Li L, Fei Z, Ren J, et al.Functional imaging of interleukin 1 beta expression in inflammatory process using bioluminescence imaging in transgenic mice[J]. BMC Immunol, 2008, 9:49.
[12] Michalova K, Bucchini D, Ripoche MA, et al.Chromosome localization of the human insulin gene in transgenic mouse lines[J]. Hum Genet, 1988, 80(3):247-252.

[1]	Jiani LIU, Jiangang LIU, Yun WEI, Hao LI, Zenggang LUO, Yi WANG, Kun LI. Pathological and Synaptic Morphological Changes of the Olfactory Bulb in APP/PS1 Model Mice at Different Ages and the Intervention Effect of Memantine [J]. Laboratory Animal and Comparative Medicine, 2022, 42(3): 177-186.
[2]	WANG Qianqian, WANG Wei, LIU Rui, WEI Zhen, LIU Chong. Factors Affecting Production Efficiency of Bacterial Artificial Chromosome Transgenic Mice [J]. Laboratory Animal and Comparative Medicine, 2021, 41(6): 535-542.
[3]	JIN Lu, LI Yan, LI Zhi-cao, WU Xi-jun, ZHOU Yan-hua, HE Zhi-xu, SHU Li-ping. Effects of Irx5a Gene Overexpression on Early Hematopoietic in Zebrafish Embryos [J]. Laboratory Animal and Comparative Medicine, 2017, 37(4): 309-314.
[4]	ZHOU Wei-min, ZHU Ke-yan, CHEN Fang-ming, CAI Yue-qing, FANG Ming-sun, Qi Yue-han, CHEN min-li. Pathological Change of Kidney in Pkd1 Knock-out Mice with Polycystic Kidney Induced by Tamoxifen [J]. Laboratory Animal and Comparative Medicine, 2017, 37(1): 11-14.
[5]	ZHANG Mei-ying, YANG Wei, WU Hong-lian, DONG Wan-wei, ZHOU Sheng-lai,YU Yang, WANG Wei, GUO Xiao-chong, ZHENG Zhi-hong. A Preliminary Study on Phenotype of 5-Lipoxygenase Transgenic Mice [J]. Laboratory Animal and Comparative Medicine, 2015, 35(4): 265-270.
[6]	LI Zhao-yang, JIANG Chuan, GUO Xiao-chong, YANG Wei, DONG Wan-wei, ZHENG Zhi-hong. Modeling of B16 Melanoma in Dicer1 Transgenic Mice [J]. Laboratory Animal and Comparative Medicine, 2015, 35(4): 294-297.
[7]	GUO Xin-bing, GONG Xiu-li, CHEN Yan-wen, FANG Yu-dan, ZHANG Jing-zhi. Viral Particle Titer and Transgene Length Affect Integration Rate of Lentiviral Vector Mediated Transgenic Mice [J]. Laboratory Animal and Comparative Medicine, 2013, 33(3): 185-189.
[8]	LI Na, WANG Wei, LI Chen-jing, ZHANG Ying-yan, ZHANG Sheng, ZHANG Hai-yan, ZHANG Zhou. Analysis on Reproductive Performance and Growth of APPswe/PS1dE9 Transgenic Mice Model for Alzheimer's Disease [J]. Laboratory Animal and Comparative Medicine, 2012, 32(3): 222-227.
[9]	SHEN Yan1，XU Chun-hua2，LI Xiang-rui3，FEI jian4，ZHOU Wen-jiang5. Comparison on Different Breeding System and Identification of C57BL/6J-HBV Transgenic Mice [J]. , 2009, 29(4): 248-253.
[10]	CHENG Ming-jun1,YU Er-kai1,GUO Fang1,WANG Yi-sen2,XU Cong-jian1. Evaluation and Establishment of Endometriosis Model by using Transgenic Mice Expressing GFP [J]. , 2007, 27(4): 219-221.
[11]	YI Xue-rui1,ZENG Yin2，ZHU Ping，ZHU Zheng-yu2,LIU Guang-zhe1，GAN Jian-hui2,YUAN You-cheng1, WANG En-hao2, ZHANG Yi-jun1. Cellular Imnune Regulation Effect of Recombinant Hepatitis B Vaccine on HBV Transgenic Mice [J]. , 2003, 23(3): 151-153.
[12]	SUN Qiang, WANG Xiao-hui, ZhANG Jing, LI Li, XUE Zheng-feng, LI Hou-da. Cryopreservation of MMTV-Wnt-1 Transgenic Mice Embryo by Using High Concentration Ethylene Glycol [J]. , 2001, 21(4): 205-208.
[13]	ZHAO Han-Fang-1, ZHANG You-Zhang-1, LIU Jun-1, CHENG Feng-1, MI Lei-2, XU Wei-Shi-2, SHI Ji-Xiang-2. mRNA Expressions of Growth Factors in Exudate Cells in Rat during Wound Healing after Partial Thickness Burn [J]. , 1998, 18(2): 77-80.