Pathological Change of Kidney in Pkd1 Knock-out Mice with Polycystic Kidney Induced by Tamoxifen

doi:10.3969/j.issn.1674-5817.2017.01.003

Abstract

Abstract: Objective To explore the change of kidney function of polycystic kidney disease (PKD) mice before and after the knockout Pkd1f/f: Cre gene induced by Tamoxifen induction. Methods The Pkd1f/f:Cre transgenic mice, Pkd1f/f:Cre wild type mice aged on 11 days were induced by Tamoxifen,the contents of serum creatinine and urea nitrogen were detected at 7 days, 14 days and 21days after induction. The body weight, kidney weight and the kidney viscera coefficient were detected after 21 day. Result After 7 days induction, the contents of serum creatinine and urea nitrogen began to rise after 14 days and 21 days induction, the parameters were significantly increased (P<0.01) After 21 days induction,the kidney coefficient of knocked out transgenic mice kidney coefficient was higher than that of wild type mice. Conclusion The renal function of Pkd1f/f:Cre transgenic mice were obviously changed by Tamoxifen induction, the kidney of transgenic mice showed symptoms of a large number of vesicles.

Key words: Polycystic kidney disease (PKD), Transgenic mice, Kidney function, Induction

CLC Number:

Q95-33

ZHOU Wei-min, ZHU Ke-yan, CHEN Fang-ming, CAI Yue-qing, FANG Ming-sun, Qi Yue-han, CHEN min-li. Pathological Change of Kidney in Pkd1 Knock-out Mice with Polycystic Kidney Induced by Tamoxifen[J]. Laboratory Animal and Comparative Medicine, 2017, 37(1): 11-14.

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Pathological Change of Kidney in Pkd1 Knock-out Mice with Polycystic Kidney Induced by Tamoxifen

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