Establishment of Vascular Restenosis Model Induced by Carotid Artery Balloon Injury in Rats

doi:10.3969/j.issn.1674-5817.2015.02.001

Abstract

Abstract: Objective To establish a simple and useful method of rat carotid artery balloon injury, providing stable and reliable animal model for studying the changes of vessels based on physical damage. Methods Rat common carotid artery, external carotid artery and internal carotid artery were isolated after anesthesia with isoflurane, the artery incision was performed in the external carotid artery, and then the 2F balloon catheter was inserted into common carotid artery in the incision, and pushed about 4 cm. Meanwhile, 0.02 mL normal saline was slightly injected to make the balloon inflate, and then the catheter was gently rotated for all the way when removing. Repeat this operation by three times to completely denude the common carotid artery intima. The changes of histomorphology in carotid artery were observed 1 day, 3 days, 7 days, 14 days, and 28 days after injury. Results Obvious neointima hyperplasia appeared over time after damage, which reached the peak at 14 days. Conclusion The improved rat carotid artery balloon injury model is established with the characteristics of simple operation, high success rate, and low rate of thrombosis.

Key words: Common carotid artery, Vascular restenosis, Balloon withdraw injury, 2F Fogarty arterial embolectomy balloon catheter, Neointimal hyperplasia

CLC Number:

Q95-33

YANG Bo, WU Bing, TANG Jun-ming, YANG Jian-ye, CAO Teng, WANG Jia-ning. Establishment of Vascular Restenosis Model Induced by Carotid Artery Balloon Injury in Rats[J]. Laboratory Animal and Comparative Medicine, 2015, 35(2): 87-91.

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