Abstract: Objective To establish the infertility mice model with the phenotype of polycystic ovary syndrome. Method Five-days-old female BALB/c mice were hypodermically injected on the neck and back with androgen testosterone propionate and were fed regularly until to adult. The vaginal epithelial cellular morphology cycle, sexual behavior, pregnancy ability, serum androgen levels, glucose tolerance, serum insulin levels and ovarian pathological changes were observed. Result The challenged female mice showed infertility with the character of refusing sexual behavior and constant vaginal epithelial cell keratinization. Both the testosterone level in serum and the basic blood glucose were higher in the mice than that in the control(P<0.05). Furthermore, polycystic ovarian follicle and cystic dilatation follicle were detected in androgen treated mice. Conclusion Hypodermic injection of postnatal female mice with testosterone propionate can sufficiently induces infertility in adult and results in loss of ovulatory cycle and abnormal ovarian pathological changes in animals. The testosterone propionate treated mouse could be used as one of animal model in the research area of polycystic ovary syndrome.

Key words: Infertility, Polycystic ovary syndrome, Animal model, BALB/c