Inhibitory Effects of Sporoderm-broken Ganoderma Lucidum Spores on T-cell lymphoma

doi:10.3969/j.issn.1674-5817.2011.06.006

Abstract

Abstract: Objective To evaluate the inhibitory effects of sporoderm-broken Ganoderma Lucidum Spores on EL-4 cell in vitro, and in vivo, the nude mice model of the subcutaneous transplanting thymic lymphoma induced by MNU was established to evaluate the inhibitory effect. Mdthods The inhibitory effects of Ganoderma Lucidum Spores on EL-4 cell were detected by MTT method in vitro. The models of the subcutaneous transplanting tumor were established by Thymus T-cell lymphoma cells induced by MNU, the inhibitory rates were observed, and the tumor tissues of the nude mice were taken with HE staining. Results With the increasing of time, the IC₅₀ values were decreased, the minimums of which were 4.76g/L after 72 hours. In vivo, the Ganoderma Lucidum spores had certain experiment therapeutic anti-tumor action, and the inhibitory rate was 45.8% with the dose of 4g/kg. The morphologic study of Thymus lymphoma cells of Garoderma Lucidum Spoers group showed more necrosis and nucleoli crumb. Conclusion Garoderma Lucidum Spoers could play inhibitory action on lymphoma cell at high concentration and high dosage.

Key words: Ganoderma lucidum Spores, T-cell lymphoma, EL-4cell, Nude mice

CLC Number:

Q95-33

CHEN Juan, YU Ying-hao. Inhibitory Effects of Sporoderm-broken Ganoderma Lucidum Spores on T-cell lymphoma[J]. Laboratory Animal and Comparative Medicine, 2011, 31(6): 426-431.

References

[1] 王筱婧, 孙莉莎, 徐江平. 灵芝孢子粉抑制人肝癌细胞生长的试验研究[J]. 贵阳医学院学报, 2006, 31(1):31-33.
[2] 梁军, 侯亚义. 破壁灵芝孢子粉诱导MCF-7细胞凋亡的机理研究[J]. 中国野生植物资源, 2006, 25(2):49-52.
[3] 李怡岚, 乔姗姗, 李国星. 灵芝粉抗肿瘤及提高机体免疫力作用的研究[J]. 中国慢性病预防与控制, 2004, 12(4):156-157.
[4] Yue GG, Fung KP, Leung PC, et al.Comparative Studies on the Immunomodulatory and Antitumor Activities of the Different Parts of Fruiting Body of Ganoderma lucidum and Ganoderma Spores[J]. Phytother. Res,2008, 22(10):1282-1291.
[5] Mosmann T.Rapid colorimetric assay for celluar growth and suvrival: application to proliferation and cytotoxieity assay[J]. J Immunol Metholds, 1983, 65(1-2):55-63.
[6] 范双翼, 余英豪, 吴在增. N-甲基亚硝基脲诱导胸腺淋巴瘤动物模型的改进.中国实验血液学杂志, 2010, 18(4):914-918.
[7] Zhang LH, Xiao PG.Prevention and treatment of experimental diabetes in mice by spore extract of Lingzhi[J]. Chin Tradit Herb Drugs(中草药), 1993, 24(5):246-247.
[8] 张卫明, 孙晓明, 吴素玲, 等. 灵芝孢子粉调节血脂作用研究[J]. 中国野生植物资源, 2001, 20(2):14-16.
[9] 王帮武, 杨新林, 张利刚, 等.灵芝酸性组份的提取分离及抑菌活性研究[J]. 北京理工大学学报, 2002, 22(1):125-128.
[10] 王明宇, 刘强, 车庆明, 等. 灵芝三萜类化合物对3种小鼠肝损伤模型的影响[J]. 药学学报, 2000, 35(5):326-329.
[11] EL-Mekkawy S, Meselhy MR, Nakamura N, et al.Anti HIV-1 and HIV-protease substances from Glanoderma lucidu[J]. Phytoche, 1998, 49(6):1651-1657.
[12] Liu X, Yuan JP, Chung CK, et al.Antitumor activity of the sporoderm-broken germinating spores of Ganoderma lucidum[J]. Cancer Lett, 2002, 182(2):155-161.
[13] Sliva D, Sedlak M, Slivova V, et al.Biologic activity of spores and dried powder from Ganoderma lucidum for the inhibition of highly invasive human breast and prostate cancer cells[J]. J Altern Complement Med, 2003, 9(4):491-497.
[14] Hong KJ, Dunn DM, Shen CL, et al.Effects of Ganoderma lucidum on apoptotic and anti-inflammatory function in HT-29 human colonic carcinoma cells[J]. Phytother Res, 2004, 18(9):768-770.
[15] 陈小君, 刘翠萍, 刘昕, 等. 萌动激活灵芝孢子粉对肿瘤组织端粒酶抑制作用的研究[J]. 中草药, 2001, 32(增刊):165-166.
[16] 陈纯, 王恒邦, 吴盈莹, 等. 灵芝及灵芝孢子提取物的抗肿瘤及对DNA拓扑异构酶作用的研究[J]. 中国药理与临床, 2008, 24(2):45-49.