Down-regulated Expression of Perilipin in Leydig Cells of Aging Male Rats

doi:10.3969/j.issn.1674-5817.2011.06.001

Abstract

Abstract: Objective To evaluate the relationship of Perilipin expression with reduced androgen synthesis in aging male rats. Methods The three-month old rats were injected every day with D-galactose (300 mg/kg) for two months to establish the model of aging rat. The levels of total testosterone (TT), superoxitase dismutase (SOD) and malondialdehyde (MDA) in serum were used as the criterion to evaluate the quality of the animal model. For measurement of differentially-expressed genes, the Leydig cells was separated by Percoll discontinuous density gradient centrifugation, cultured for three day and then used for preparation of total RNA. Gene expression profile in the aging Leydig cells was studied in by cDNA microarray technique plus validation of screened genes by RT-PCR. Results In D-galactose-injected rats, the serum levels of MDA and SOD were significantly higher than those of the control rats. However, the serum level of TT was significantly lower in the rats injected with D-galactose than that of the control rats. Expression of hundreds of genes changed in the Leydig cells of aging rats, compared to that in the control, screened by cDNA microarray technique. Among these genes, Perilipin expression was selected for validation by RT-PCR technique, and our data indicated that levels of both Perilipin A and Perilipin B expression were significantly reduced in the aging Leydig cells. Conclusion The aging male rats were well established. Down-regulated expression of Perilipin may result in a reduced androgen synthesis in Leydig cells of aging male rats.

Key words: PADAM, D-galactose, Leydig cell, Gene chip, RT-PCR, Perilipin

CLC Number:

Q95-33

HE Hua, LIU Yuan-yuan, LI Yu-hua, YUAN Wei, LI Runsheng. Down-regulated Expression of Perilipin in Leydig Cells of Aging Male Rats[J]. Laboratory Animal and Comparative Medicine, 2011, 31(6): 405-409.

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