Establishment and Validation of a Guinea Pig Model of Ear Psoriasis Induced by Propranolol Hydrochloride

doi:10.12300/j.issn.1674-5817.2026.011

Abstract

Abstract:

Objective To induce psoriasis in the ears of guinea pigs using 5% propranolol hydrochloride emulsion, establish a stable local psoriasis animal model, and verify its application value in drug efficacy evaluation. Methods Twenty-nine 6-8 week-old male Hartley guinea pigs of normal strain were randomly grouped according to their body weight. Nine guinea pigs were selected as the control group, and the remaining animals were the model group. The model group was treated with 5% propranolol hydrochloride emulsion on the right ear back, 4 times a day for 4 consecutive days. The criteria for model formation were an ear thickness difference > 0.05 mm and a Psoriasis-like skin lesion area and disease severity index (PASI) score > 3. The model animals were evenly and randomly grouped based on the ear thickness difference as the main indicator, and with PASI score and body weight as secondary indicators, into the model group, the 1.6 mg/kg methotrexate group, and were given continuous treatment for 10 days. During the experiment, changes in skin lesions, body weight, ear thickness difference, and PASI score were observed. At the end of the experiment, samples were taken for detection of ear weight, spleen index, ear tissue pathological changes, and mast cell infiltration degree indicators. Results The animals in the modeling group showed redness, swelling, and thickening of psoriasis-like skin lesions on the right ear 2 day after modeling, accompanied by obvious scratching behavior. The peak period was 4-9 days. The ear skin was significantly red, thick, and had more scales. The PASI score, ear thickness difference, ear swelling, spleen index, tissue pathological changes, and mast cell infiltration degree indicators of the modeling group were significantly higher than those of the normal group. Methotrexate could significantly improve the above detection indicators. Conclusion This study successfully established a stable and repeatable psoriasis model on the ear of guinea pigs induced by propranolol hydrochloride, which is suitable for the evaluation of the efficacy of local psoriasis.

Key words: Psoriasis, Guinea pig, Propranolol hydrochloride, Animal model, Pharmacological efficacy evaluation

CLC Number:

R-332

MENG Feibiao,WANG Linyi,ZHANG Wenqiang,et al. Establishment and Validation of a Guinea Pig Model of Ear Psoriasis Induced by Propranolol Hydrochloride[J]. Laboratory Animal and Comparative Medicine. DOI: 10.12300/j.issn.1674-5817.2026.011.

Figures/Tables 9

References 25

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比例 Ratio	组分 Component	重量（g） Weight (g)	百分比（%） Percentage (%)
油相Oil phase	硬脂酸Stearic acid	1.4	14
油相Oil phase	液体石蜡Liquid paraffin	2.4	24
水相Aqueous phase	三乙醇胺Triethanolamine	0.3	3
	甘油Glycerol	0.9	9
	蒸馏水Distilled water	4.5	45
药物Medicine	盐酸普萘洛尔Propranolol hydrochloride	0.5	5
总量Total quantity		10	100

比例 Ratio	组分 Component	重量（g） Weight (g)	百分比（%） Percentage (%)
油相Oil phase	硬脂酸Stearic acid	1.4	14
油相Oil phase	液体石蜡Liquid paraffin	2.4	24
水相Aqueous phase	三乙醇胺Triethanolamine	0.3	3
	甘油Glycerol	0.9	9
	蒸馏水Distilled water	4.5	45
药物Medicine	盐酸普萘洛尔Propranolol hydrochloride	0.5	5
总量Total quantity		10	100

皮肤泛红程度 Degree of skin redness	鳞屑 Scale	增厚程度 Degree of thickening	严重程度 Different degrees of severity	转换评分 Conversion score
无泛红现象 No redness at all	耳部表面无可见鳞屑	未见增厚	无症状	0
耳部皮肤呈淡红色 The skin on the ear is a light red color.	部分皮损表面完全或覆有鳞屑，以细微鳞屑为主	略有增厚，但尚属轻微范围	轻度	1
耳部皮肤呈红色 The skin on the ear is red.	大多数皮损表面完全或不完全覆有鳞屑，鳞屑呈片状	呈中等程度增厚，变化较为显著	中度	2
耳部皮肤呈深红色 The skin on the ear is a deep red color	几乎所有皮损表面覆有鳞屑，较厚成层	明显增厚，变化突出	重度	3
耳部皮肤红色极深 The skin on the ear is extremely red	全部皮损表面均覆有鳞屑，鳞屑很厚成层	异常增厚，已达严重程度	极重度	4

皮肤泛红程度 Degree of skin redness	鳞屑 Scale	增厚程度 Degree of thickening	严重程度 Different degrees of severity	转换评分 Conversion score
无泛红现象 No redness at all	耳部表面无可见鳞屑	未见增厚	无症状	0
耳部皮肤呈淡红色 The skin on the ear is a light red color.	部分皮损表面完全或覆有鳞屑，以细微鳞屑为主	略有增厚，但尚属轻微范围	轻度	1
耳部皮肤呈红色 The skin on the ear is red.	大多数皮损表面完全或不完全覆有鳞屑，鳞屑呈片状	呈中等程度增厚，变化较为显著	中度	2
耳部皮肤呈深红色 The skin on the ear is a deep red color	几乎所有皮损表面覆有鳞屑，较厚成层	明显增厚，变化突出	重度	3
耳部皮肤红色极深 The skin on the ear is extremely red	全部皮损表面均覆有鳞屑，鳞屑很厚成层	异常增厚，已达严重程度	极重度	4

病变程度 Degree of lesion	组织学改变 Histological changes	评分（分） Score(points)
正常Normal	未观察到组织学改变或组织结构基本正常	0
轻微Slight	病理损伤范围零星可见，不构成区域性	0.5
轻度Mild	灶性、多灶性或散在分布的损伤，损伤范围在100倍镜下可见1/3-1个视野	1
中度Moderate	灶性、多灶性或散在分布的损伤，损伤范围在100倍镜下可见1-3个视野	2
重度Severe	灶性、多灶性或散在分布的损伤，损伤范围在100倍镜下可见3个视野以上	3