Abstract:

Objective To assess the ability of the inspection institutions of laboratory animals and to improve their detection levels through the implementation of the proficiency testing for the detection ability of Bordetella bronchiseptica, Pasteurella pneumotropica, Pasteurella multocida and Corynebacterium kutscheri in laboratory animals. Methods According to the proficiency testing scheme approved by China National Accreditation Service for Conformity Assessment (CNAS), the two projects, NIFDC-PT-309 and NIFDC-PT-363 in 2021 and 2022 were set up, respectively. The samples of respiratory tract pathogens in laboratory animal were prepared by freeze-drying at low temperature. The samples were randomly grouped and numbered through the CNAS platform and issued to participating units after passing the homogeneity and stability tests. A work instruction was attached with each sample set. The inspection reports and original records were required to be submitted within the specified time. If the feedback results were consistent with the sample setting, the results were deemed as satisfactory, and if the feedback results were inconsistent or not submitted on time, the results were deemed as unsatisfactory. Results There were 29 laboratories joined to NIFDC-PT-309, 27 laboratories (93.1%) achieved satisfactory test results. There were 30 laboratories joined to NIFDC-PT-363, the satisfaction rate is 100%. Conclusion Laboratory animal quality inspection institutions have the ability to detect respiratory pathogens, which gets steadily improved through the ability verification activities.

Key words: Bordetella bronchiseptica, Pasteurella pneumotropica, Pasteurella multocida, Corynebacterium kutscheri, Proficiency testing