Pathological Diagnosis of Systemic Amyloidosis in a New Zealand White Rabbit

doi:10.12300/j.issn.1674-5817.2024.092

Abstract

Abstract:

Objective To provide a reference for the diagnosis of amyloidosis in experimental animals through the pathological diagnosis of systemic amyloidosis in a case of a New Zealand white rabbit. Methods In a 6-month repeated ocular toxicity study, an abnormal finding was noted during the routine gross anatomical examination of one New Zealand white rabbit. Its organs were prepared as paraffin sections and stained with hematoxylin-eosin (HE) staining and Congo red staining. The histopathological features were observed under optical and polarized light microscopy. Results Gross anatomical examination of the animal revealed an enlarged spleen and changes in the color and texture of the lung. HE staining showed that the splenic tissue structure was destroyed, the white pulp of the spleen was surrounded by dense amyloid deposition in the form of nodular rings, along with pressure atrophy of the white pulp. Amyloid deposits were also observed in the submandibular lymph nodes, mesenteric lymph nodes, ileum, sacculus rotundus, vermiform appendix, jejunum, cecum, and rectum. Congo red staining showed that the amyloid deposition in the affected organs appeared salmon-pink, and exhibited characteristic apple green birefringence under polarized light microscopy.Conclusion The histo-pathological features of the New Zealand white rabbit are consistent with the diagnostic characteristics of systemic amyloidosis.

Key words: Amyloidosis, Spleen, Pathological morphology, New Zealand white rabbit

CLC Number:

S852.31

LIN Qingqing,DAI Jinlong,CHEN Zhisen,et al. Pathological Diagnosis of Systemic Amyloidosis in a New Zealand White Rabbit[J]. Laboratory Animal and Comparative Medicine, 2024, 44(6): 695-699. DOI: 10.12300/j.issn.1674-5817.2024.092.

Figures/Tables 4

Figure 1 Spleen of a New Zealand white rabbit suspected of systemic amyloidosis

Figure 2 General optical microscopic observation of affected organs in a New Zealand white rabbit with systemic amyloidosisNote： A-F show amyloid deposition in the spleen at low magnification （×40） and high magnification （×100）， submandibular lymph node （×200）， mesenteric lymph node （×200）， rectum （×200） and ileum （×200）； G-L show histological morphology of spleen， submandibular lymph nodes， mesenteric lymph nodes， rectum， and ileum under the same magnification after Congo red staining. Scale bars： 600 μm （A，G）， 300 μm（B，H）， 200 μm（C-F，I-L）.

Figure 3 Polarized light microscopy of affected organ tissue in a New Zealand white rabbit with systemic amyloidosis （Congo red staining， ×200）Note：A， spleen； B， submandibular lymph nodes； C， rectum； D， ileum. Scale bar： 100 μm （A-D）.

Figure 4 Histopathology of other organs of a New Zealand white rabbit with systemic amyloidosis （HE staining，×40）Note：A， Severe diffuse inflammation of the lungs； B， Thymic atrophy； C， Diffuse increase in cellularity in the bone marrow cavity； D， Bone marrow micrograph of normal control animals at the same time point. All scale bars in the figure are 600 μm （A-D）.

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