Survey Report Analysis on Parasitic and Microbial Quality of Laboratory Animals in Sichuan Province, 2017-2023

doi:10.12300/j.issn.1674-5817.2024.057

Abstract

Abstract:

Objective To provide reference for further strengthening the management of laboratory animals in Sichuan Province by reviewing and analyzing the results of parasitic and microbial quality inspections of laboratory animals from 2017 to 2023. Methods Sichuan Province has 31 licensed laboratory animal production units, with the main species including mice, rats, guinea pigs, rabbits, dogs, monkeys, and pigs. Sampling inspections and reports were conducted for units with laboratory animal production qualifications in accordance with current national and local standards for laboratory animal classification. The quality inspection results for various laboratory animals in Sichuan Province from 2017 to 2023 were analyzed. Results With the exception of 2018, annual quality inspections of laboratory animals were conducted every year between 2017 and 2023. Mice: In 2017, positive results for helminths, flagellates, Salmonella, murine hepatitis virus antibodies, and murine pneumonia virus antibodies were detected, with a pass rate of only 42.9%. In 2019, Staphylococcus aureus and Klebsiella pneumoniae were detected, with a pass rate of 86.7%. In 2021, Sendai virus antibodies were detected, yielding a pass rate of 85.7%.The pass rate in 2020, 2022, and 2023 was 100%. Rats: In 2017, positive results were found for helminths, mycoplasma antibodies, Staphylococcus aureus, Sendai virus antibodies, murine pneumonia virus antibodies, rat parvovirus RV strain antibodies, rat parvovirus (H-1) strain antibodies, and rat coronavirus antibodies, with a pass rate of 40.0%. In 2019, mycoplasma antibodies, Staphylococcus aureus and Klebsiella pneumoniae were detected, with a pass rate of 35.0%. No positive indicators were detected in 2020. In 2021, Sendai virus antibodies and rat parvovirus RV strain antibodies were detected, with a pass rate of 50.0%. In 2022, positive results for rat parvovirus RV strain antibodies were found, yielding a pass rate of 87.5%. In 2023, Pasteurella pneumotropica and Klebsiella pneumoniae were detected, with a pass rate of 85.7%. Dogs: In 2017 and 2019, the antibody titers for rabies virus and canine distemper virus were below the required standard, with pass rates of 33.3% and 20.0%, respectively. In 2020 and 2022, the pass rate was 100%. Guinea pigs (general grade): In 2019, positive results for Toxoplasma antibodies were detected, with a pass rate of 80.0%. In all other years, the pass rate was 100%. Monkeys: In 2019, positive results for Toxoplasma gondii and rhesus herpesvirus type I antibodies were found, with a pass rate of 87.5%.In 2020 and 2022, rhesus herpesvirus type I antibodies were positively detected, yielding pass rates of 93.3% and 97.5%, respectively. The pass rates in 2021 and 2023 were 100%. Clean guinea pigs, rabbits and pigs all passed the inspection each year. Conclusion Issues related to the parasitic and microbial quality of laboratory animals persist in Sichuan Province. Supervision and sampling inspections have proven to be effective in identifying these issues promptly, serving as a critical measure to ensure the quality of laboratory animals. The results of these inspections offer valuable data to support the healthy development of the laboratory animal industry in Sichuan Province.

Key words: Sichuan Province, Laboratory animals, Quality inspection, Parasites, Microorganisms

CLC Number:

S853.32

LIU Lida,CHEN Bing,XIE Na,et al. Survey Report Analysis on Parasitic and Microbial Quality of Laboratory Animals in Sichuan Province, 2017-2023[J]. Laboratory Animal and Comparative Medicine, 2024, 44(6): 654-660. DOI: 10.12300/j.issn.1674-5817.2024.057.

Figures/Tables 3

References 13

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年份 Year	抽检单位/家 Number of units	抽检动物数量/只^# Number of laboratory animals^#
年份 Year	抽检单位/家 Number of units	小鼠^a Mouse^a	大鼠^a Rat^a	豚鼠^b Guinea pig^b	豚鼠^c Guinea pig^c	兔^b Rabbit^b	犬^b Dog^b	猴^b Monkey^b	小型猪^b Miniature pig^b	总数 Total
2017	8	35 (7)	25 (5)	NA	10 (2)	NA	15 (3)	NA	NA	85
2019	13	30 (6)	20 (4)	10 (2)	10 (2)	10 (2)	10 (2)	40 (4)	NA	130
2020	9	25 (5)	25 (5)	NA	NA	NA	15 (3)	45 (3)	NA	110
2021	14	35 (7)	20 (4)	10 (2)	10 (2)	NA	NA	40 (4)	20 (4)	135
2022	17	50 (10)	40 (8)	NA	NA	NA	10 (2)	40 (4)	20 (4)	160
2023	14	50 (10)	35 (7)	10 (2)	10 (2)	5 (1)	NA	50 (4)	NA	160
Total	75	225	165	30	40	15	50	215	40	780

年份 Year	抽检单位/家 Number of units	抽检动物数量/只^# Number of laboratory animals^#
年份 Year	抽检单位/家 Number of units	小鼠^a Mouse^a	大鼠^a Rat^a	豚鼠^b Guinea pig^b	豚鼠^c Guinea pig^c	兔^b Rabbit^b	犬^b Dog^b	猴^b Monkey^b	小型猪^b Miniature pig^b	总数 Total
2017	8	35 (7)	25 (5)	NA	10 (2)	NA	15 (3)	NA	NA	85
2019	13	30 (6)	20 (4)	10 (2)	10 (2)	10 (2)	10 (2)	40 (4)	NA	130
2020	9	25 (5)	25 (5)	NA	NA	NA	15 (3)	45 (3)	NA	110
2021	14	35 (7)	20 (4)	10 (2)	10 (2)	NA	NA	40 (4)	20 (4)	135
2022	17	50 (10)	40 (8)	NA	NA	NA	10 (2)	40 (4)	20 (4)	160
2023	14	50 (10)	35 (7)	10 (2)	10 (2)	5 (1)	NA	50 (4)	NA	160
Total	75	225	165	30	40	15	50	215	40	780

动物 Animal	检出病原体 Pathogens		不同年份的检出阳性率/% Positive rate in different years/%
动物 Animal	类别 Category	名称 Name	2017	2019	2020	2021	2022	2023
SPF级小鼠 SPF mouse	寄生虫	蠕虫	17.1 (4a、2f)	0	0	0	0	0
		鞭毛虫	8.6 (3a)	0	NA	NA	0	0
	病原菌	沙门菌	5.7 (2d)	0	0	0	0	0
		金黄色葡萄球菌	0	10.0 (1b、2c)	0	NA	NA	NA
		肺炎克雷白杆菌	0	3.3 (1a)	0	0	NA	0
	病毒	小鼠肝炎病毒抗体	57.1 (5a、5c、5d、5f)	0	0	0	NA	0
		小鼠肺炎病毒抗体	20.0 (2a、5f)	0	0	0	0	0
		仙台病毒抗体	0	0	0	14.3 (5h)	0	0
SPF级大鼠 SPF rat	寄生虫	蠕虫	24.0 (1a、2c、3f)	0	0	0	0	0
	病原菌	支原体抗体	20.0 (5a)	25.0 (5h)	0	0	0	0
		金黄色葡萄球菌	16.0 (2b、2f)	35.0 (2b、5c)	0	NA	NA	NA
		嗜肺巴斯德杆菌	0	0	0	NA	NA	11.4 (4a)
		肺炎克雷白杆菌	0	5.0 (1a)	0	0	NA	2.9 (1l)
	病毒	仙台病毒抗体	4.0 (1a)	0	0	25.0 (5h)	0	0
		小鼠肺炎病毒抗体	20.0 (5a)	0	0	NA	0	NA
		大鼠细小病毒RV株抗体	48.0 (5a、5c、2f)	0	0	25.0 (5a)	13 (5a)	0
		大鼠细小病毒H-1株抗体	32.0 (4a、1c、3f)	0	0	NA	0	0
		大鼠冠状病毒抗体	20.0 (5a)	0	NA	0	NA	NA
普通级兔 Rabbit	寄生虫	弓形虫抗体	NA	0	NA	NA	NA	0
普通级犬 Dog	病毒	狂犬病毒抗体	40.0 (3c、3e、0g)	20.0 (1e、1g)	100	NA NA	100	NA NA
普通级犬 Dog	病毒	犬瘟热病毒抗体	73.3 (2c、5e、4g)	70 (3e、4g)	100	NA NA	100	NA NA
普通级豚鼠 Guinea pig	寄生虫	弓形虫抗体	NA	20.0 (2j)	NA	0	NA	0
普通级猴 Monkey	寄生虫	弓形虫抗体	NA NA	5.0 (1e、1i)	0	0	0	0
普通级猴 Monkey	病毒	猕猴疱疹病毒I型（B病毒）抗体	NA NA	12.5 (3e、2i)	6.7 (3e)	0	2.5 (1e)	0

动物 Animal	检出病原体 Pathogens		不同年份的检出阳性率/% Positive rate in different years/%
动物 Animal	类别 Category	名称 Name	2017	2019	2020	2021	2022	2023
SPF级小鼠 SPF mouse	寄生虫	蠕虫	17.1 (4a、2f)	0	0	0	0	0
		鞭毛虫	8.6 (3a)	0	NA	NA	0	0
	病原菌	沙门菌	5.7 (2d)	0	0	0	0	0
		金黄色葡萄球菌	0	10.0 (1b、2c)	0	NA	NA	NA
		肺炎克雷白杆菌	0	3.3 (1a)	0	0	NA	0
	病毒	小鼠肝炎病毒抗体	57.1 (5a、5c、5d、5f)	0	0	0	NA	0
		小鼠肺炎病毒抗体	20.0 (2a、5f)	0	0	0	0	0
		仙台病毒抗体	0	0	0	14.3 (5h)	0	0
SPF级大鼠 SPF rat	寄生虫	蠕虫	24.0 (1a、2c、3f)	0	0	0	0	0
	病原菌	支原体抗体	20.0 (5a)	25.0 (5h)	0	0	0	0
		金黄色葡萄球菌	16.0 (2b、2f)	35.0 (2b、5c)	0	NA	NA	NA
		嗜肺巴斯德杆菌	0	0	0	NA	NA	11.4 (4a)
		肺炎克雷白杆菌	0	5.0 (1a)	0	0	NA	2.9 (1l)
	病毒	仙台病毒抗体	4.0 (1a)	0	0	25.0 (5h)	0	0
		小鼠肺炎病毒抗体	20.0 (5a)	0	0	NA	0	NA
		大鼠细小病毒RV株抗体	48.0 (5a、5c、2f)	0	0	25.0 (5a)	13 (5a)	0
		大鼠细小病毒H-1株抗体	32.0 (4a、1c、3f)	0	0	NA	0	0
		大鼠冠状病毒抗体	20.0 (5a)	0	NA	0	NA	NA
普通级兔 Rabbit	寄生虫	弓形虫抗体	NA	0	NA	NA	NA	0
普通级犬 Dog	病毒	狂犬病毒抗体	40.0 (3c、3e、0g)	20.0 (1e、1g)	100	NA NA	100	NA NA
普通级犬 Dog	病毒	犬瘟热病毒抗体	73.3 (2c、5e、4g)	70 (3e、4g)	100	NA NA	100	NA NA
普通级豚鼠 Guinea pig	寄生虫	弓形虫抗体	NA	20.0 (2j)	NA	0	NA	0
普通级猴 Monkey	寄生虫	弓形虫抗体	NA NA	5.0 (1e、1i)	0	0	0	0
普通级猴 Monkey	病毒	猕猴疱疹病毒I型（B病毒）抗体	NA NA	12.5 (3e、2i)	6.7 (3e)	0	2.5 (1e)	0

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