Observing on Laboratory Animal Welfare Related Indexes in Chronic Stress Rats

doi:10.3969/j.issn.1674-5817.2018.03.008

Abstract

Abstract: Objective To observe the effects of chronic stress on animal welfare related indexes in rats.Methods Eight weeks old SPF SD rats were subjected with chronic stress by electric shock observing.Body weight gain and blood pressure of the rats were recorded throughout the experiment.The expressions of mRNA and protein of nuclear factor-κB (NF-κB) p65 in liver cells were measured by real-time quantitative PCR and immunohistochemical method respectively when the experiment ended.Interleukin-2 (IL-2),epinephrine (EPI),norepinephrine (NE),adrenocorticotropic hormone (ACTH) and corticosterone (CORT) in blood were measured by enzyme-linked immunosorbent assay (ELISA).Results Related indexes of group test were compared with group control,the body weight gain were lower and blood pressure were higher (P<0.05).The expressions of mRNA and protein of NF-κB p65 were higher (P<0.05).The indexes of NE,EPI,ACTH and CORT were higher and IL-2 got lower (P<0.05).Conclusion The animal welfare would be infected by chronic stress,and should be paid attention to during the animal experiment studies.

Key words: Animal welfare, Nuclear factor-κB (NF-κB), Chronic stress

CLC Number:

Q95-33

LIANG Lei, DONG Min, YOU Jin-wei, HU Wen-juan, CHEN Li, YUN Shi-feng. Observing on Laboratory Animal Welfare Related Indexes in Chronic Stress Rats[J]. Laboratory Animal and Comparative Medicine, 2018, 38(3): 207-211.

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