Abstract: Objective To establish a model of rat skin wounds which was covered with the genetically engineering membrane planted by those integrated with hVEGF recombinant plasmid. Method Wounds with full-thickness cutaneous defect were produced on the dorsum of the SD rats. The rats were randomly divided into four groups. For the rats in the experimented group, the wounds were covered with hVEGF genetically engineering membrane. For the three control groups, the wounds were covered with membrane planted by the fibroblast integrated with blank plasmid, blank membrane and Tegaderm mem-brane respectively. The specimens were obtained at the 3,7,14 and 29 days after injury, and examined histopathologically. Results Compared to that of Control groups, the hyperplasia of fibroblast and new capillary vessel in rat skin wounds were increased at all different time after injury in experiment group.Conclusion Vasculogenesis and granulation tissue growth were accelerated in skin wound which was covered with the genetically engineering membrane planted by those integrated with hVEGF recombinant plasmid.

Key words: hVEGF, Granulation tissue, Wound healing