Investigation on Current Biosafety Management Status in Laboratory Animal Institutions in Hunan Province

doi:10.12300/j.issn.1674-5817.2023.129

Abstract

Abstract:

Objective This study aimed to investigate the current status of biosafety management in laboratory animal institutions in Hunan Province, identify management shortcomings, and provide targeted recommendations for improvement. Methods A questionnaire survey was conducted in July 2023 among 40 laboratory animal institutions in Hunan Province, covering topics such as the establishment of laboratory management system and its overall operation status, emergency management for incidents, medical waste disposal, animal transportation, personnel practitioner management, environmental disinfection, and equipment maintenance. The awareness of biosafety, animal bite incidents, and vaccination history was surveyed from at least one animal feeder or experimental operation practitioner from each institution, totaling 50 practitioners. Through literature review and comparative analysis, this paper analyzed the current status of biosafety management in production, transportation, and use of laboratory animals in Hunan Province from aspects such as biosafety management systems, personnel training, disinfection of environmental facilities and waste disposal, and provided recommendations. Results Among the 40 laboratory animal institutions in Hunan Province,39 institutions (97.5%) had established laboratory management systems, 37 (92.5%) had emergency response plans for incidents of to transport laboratory animals, 15 (37.5%) had complete records of emergency drills for laboratory animal biosafety, and 39 (97.5%) had established protocols for carcass and waste management. Moreover, 26 institutions (65.0%) had laboratory biosafety risk assessment reports. 4 out of 8 (50.0%) production units had emergency plans for animal transportation, with 7 (87.5%) using special vehicles to transport laboratory animals. 2 (4.0%) of the 50 practitioners were unaware of emergency response plans, 6 participants (12.0%) did not consistently wear personal protective equipment, and 38 participants (76.0%) had been bitten or scratched by laboratory animals. The results of the survey showed that emergency management, transportation management and personnel management of laboratory animal incidents still need improvement in Hunan Province. Relevant functional departments should strengthen supervision and management efforts, establish local regulations for the transportation management of laboratory animals, ensure strict compliance with relevant documents, conduct emergency drills in all units, and strengthen training for practitioners. Conclusion While daily management practices in the 40 laboratory animal institutions in Hunan Province are generally satisfactory, there remains a need for further improvement in emergency management, transportation management, personnel management, etc. Gradually enhancing the level of laboratory animal safety management is essential to collectively safeguard public health safety.

Key words: Laboratory animals, Biosafety, Safety management, Hunan Province

CLC Number:

Q95-33

Meitong LIU,Zhang CHEN,Zhaoqiang ZHANG,et al. Investigation on Current Biosafety Management Status in Laboratory Animal Institutions in Hunan Province[J]. Laboratory Animal and Comparative Medicine, 2024, 44(2): 202-208. DOI: 10.12300/j.issn.1674-5817.2023.129.

Figures/Tables 2

References 9

1	胡小林. 实验动物生物安全风险及管理对策[J]. 畜牧兽医科学(电子版), 2019(6):26-27. DOI:10.3969/j.issn.2096-3637.2019.06.015 .
	HU X L. Biosafety risks and management countermeasures of experimental animals[J]. Graziery Vet Sci Electron Version, 2019(6):26-27. DOI:10.3969/j.issn.2096-3637.2019.06.015 .
2	马春峰, 郭振东, 汤文庭. 动物生物安全实验室常见生物危害及控制措施[J]. 畜牧与兽医, 2019,51(9):119-124.
	MA C F, GUO Z D, TANG W T. Biohazards in and control measures for the animal biosafety laboratory[J]. Anim Husb Vet Med, 2019,51(9):119-124.
3	汪文琦, 刘兰, 樊世勇, 等. 甘肃省实验动物生物安全应急管理探索与实践[J]. 特种经济动植物, 2023, 26(1):166-169. DOI: 10.3969/j.issn.1001-4713.2023.01.054 .
	WANG W Q, LIU L, FAN S Y, et al. Exploration and practice of emergency management of laboratory animal biosafety in Gansu Province[J]. Spec Econ Anim Plants, 2023, 26(1):166-169. DOI: 10.3969/j.issn.1001-4713.2023.01.054 .
4	王帝, 翟璐. 东北农业大学28名师生因动物实验感染严重传染病[EB/OL]. (2011-09-03)[2023-09-10]. http://zqb.cyol.com/html/2011-09/03/nw.D110000zgqnb_20110903_3-03.htm.
	WANG D, ZHAI L.Twenty-eight teachers and students in Northeast Agricultural University were infected with severe infectious diseases due to animal experiments [EB/OL]. (2011-09-03)[2023-09-10]. http://zqb.cyol.com/html/2011-09/03/nw.D110000zgqnb_20110903_3-03.htm.
5	WANG W L, QI W J, LIU J Y, et al. First human infection case of monkey B virus identified in China, 2021[J]. China CDC Wkly, 2021, 3(29):632-633. DOI: 10.46234/ccdcw2021.154 .
6	黄丽, 曾颖, 常伟. 湖南省实验动物资源支撑生物医药科技创新与产业发展调研报告(2022年)[J]. 中南药学, 2022, 20(8):1743-1746. DOI: 10.7539/j.issn.1672-2981.2022.08.005 .
	HUANG L, ZENG Y, CHANG W. Investigation report on biomedical science and technology innovation and industrial development supported by laboratory animal resources in Hunan Province (2022)[J]. Cent South Pharm, 2022, 20(8):1743-1746. DOI: 10.7539/j.issn.1672-2981.2022.08.005 .
7	巩薇, 贺争鸣, 李根平. 北京市实验动物突发重大事件应急预案的制定[J]. 实验动物科学, 2008, 25(1):29-33. DOI: 10.3969/j.issn.1006-6179.2008.01.009 .
	GONG W, HE Z M, LI G P. Formulation of emergency plan for laboratory animals in Beijing[J]. Lab Anim Sci, 2008, 25(1):29-33. DOI: 10.3969/j.issn.1006-6179.2008.01.009 .
8	陆建玲, 单斌, 孙兴莲, 等. 江苏省突发实验动物生物安全事件应急预案的编制[J]. 实验动物与比较医学, 2015, 35(6):510-514. DOI: 10.3969/j.issn.1674-5817.2015.06.016 .
	LU J L, SHAN B, SUN X L, et al. Compilation of emergency plan for sudden laboratory animal biosafety events in Jiangsu Province[J]. Lab Anim Comp Med, 2015, 35(6):510-514. DOI: 10.3969/j.issn.1674-5817.2015.06.016 .
9	国家认证认可监督管理委员会. 实验动物运输管理规范: RB/T 062—2021[S]. 北京中国标准出版社, 2022.
	National Certification and Accreditation Administration Regulatory Commission. Code for management of transportation of laboratory animals:RB/T 062-2021[S]. Beijing: Standards Press of China, 2022.

调查内容 Survey contents	非常了解 Know very well	一般了解 Know well	完全不了解 Do not know
是否知晓国家/湖南省实验动物相关法律法规及行业标准？ Are you familiar with national/Hunan laws and regulations on laboratory animals and industry standards?	15 （30.0）	33 （66.0）	2 （4.0）
是否了解实验动物常见的人兽共患病？ Are you familiar with common zoonoses in laboratory animals?	17 （34.0）	31 （62.0）	2 （4.0）
是否了解实验动物气溶胶的产生及危害？ Are you familiar with the production of aerosols and their hazards in laboratory animals?	18 （36.0）	26 （52.0）	6 （12.0）
是否了解本单位实验动物应急预案或处置程序？ Are you familiar with the emergency plan or procedures for handling laboratory animals in your institution?	27 （54.0）	21 （42.0）	2 （4.0）
如被实验动物抓伤或咬伤，是否知晓如何处理？ Do you know what to do if you are scratched or bitten by a laboratory animal?	39 （78.0）	10 （20.0）	1 （2.0）
如发生医疗废弃物意外流失/泄露/扩散，是否知晓如何处理？ Do you know how to deal with the accidental spill/leakage/diffusion of medical waste?	25 （50.0）	17 （34.0）	8 （16.0）
是否知晓防控病媒生物进入实验动物设施的处理方式？ Are you familiar with the management of vector control in laboratory animal facilities?	28 （56.0）	17 （34.0）	5 （10.0）
如发生实验动物逃逸，是否知晓如何处理？ Do you know what to do if animals escape from the laboratory?	25 （50.0）	20 （40.0）	5 （10.0）
如发生实验动物未知原因大量死亡，是否知晓如何处理？ Do you know what to do if a large number of laboratory animals die from unknown causes?	25 （50.0）	20 （40.0）	5 （10.0）

调查内容 Survey contents	非常了解 Know very well	一般了解 Know well	完全不了解 Do not know
是否知晓国家/湖南省实验动物相关法律法规及行业标准？ Are you familiar with national/Hunan laws and regulations on laboratory animals and industry standards?	15 （30.0）	33 （66.0）	2 （4.0）
是否了解实验动物常见的人兽共患病？ Are you familiar with common zoonoses in laboratory animals?	17 （34.0）	31 （62.0）	2 （4.0）
是否了解实验动物气溶胶的产生及危害？ Are you familiar with the production of aerosols and their hazards in laboratory animals?	18 （36.0）	26 （52.0）	6 （12.0）
是否了解本单位实验动物应急预案或处置程序？ Are you familiar with the emergency plan or procedures for handling laboratory animals in your institution?	27 （54.0）	21 （42.0）	2 （4.0）
如被实验动物抓伤或咬伤，是否知晓如何处理？ Do you know what to do if you are scratched or bitten by a laboratory animal?	39 （78.0）	10 （20.0）	1 （2.0）
如发生医疗废弃物意外流失/泄露/扩散，是否知晓如何处理？ Do you know how to deal with the accidental spill/leakage/diffusion of medical waste?	25 （50.0）	17 （34.0）	8 （16.0）
是否知晓防控病媒生物进入实验动物设施的处理方式？ Are you familiar with the management of vector control in laboratory animal facilities?	28 （56.0）	17 （34.0）	5 （10.0）
如发生实验动物逃逸，是否知晓如何处理？ Do you know what to do if animals escape from the laboratory?	25 （50.0）	20 （40.0）	5 （10.0）
如发生实验动物未知原因大量死亡，是否知晓如何处理？ Do you know what to do if a large number of laboratory animals die from unknown causes?	25 （50.0）	20 （40.0）	5 （10.0）

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