Abstract: Objective To prepare successful, stable and optimized insulin resistance combined with hyperuricemia (IR-HUA) models, the preparation methods of IR-HUA model, HUA model and IR model in rats were compared. Method Totally 40 male SD rats were randomly divided into the normal control group (A), the HUA model group (B), the IR-HUA model group (C), and the IR model group (D). The group A and group B were fed with the ordinary feed and drinking water, the group C and group D were fed with the high fat feed and high sugar diet and drinking 10% fructose water. During 8 weeks for modeling, at sixth weeks, rats in group B and group C were fed with yeast extract (16 g/kg/per day) 21d, At the end of sixth, eighth week, the observation index were detected. Result At the eighth weekend of the experiment, compared with group A, the concentrations of serum uric acid (SUA) and urine uric acid (UUA), total uric acid excretion and the fraction excretion of uric acid (FEUA) in group B increased; The levels of fasting insulin (FINS) and HOMA-IR index, and urination volume in group C decreased; the concentration of UUA and FEUA in group C increased; the levels of Lee 's index, FINS, urination volume,and total uric acid excretion in group D declined, the concentrations of fasting plasma glucose(FPG), UUA, serum creatinine (SCr) and uric creatinine (UCr) in group D increased. Conclusion HUA model could be prepared in SD rats by fed with yeast extract 16 g/kg/per day for 21d. The IR model in SD rats induced by high fat and high sugar diet for 8 weeks was not ideal, it could be due to the shorter molding time. The rat model showed no obvious characteristics of IR. The preparation method of IR-HUA model needs to be further improved. In addition, high-fat and high-sugar diet can reduce the volume of urine and the excretion of uric acid in rats, and reduce the secretion of insulin in rats, leading to the increase of FPG.

Key words: Insulin resistance combined with Hyperuricemia (IR-HUA), Fasting plasma glucose (FPG), Animal model