Abstract: Objective To investigate the estrogen-like activity and endocrinic interference effects of Bisphenol A and 4-Nonylphenol to Juvenile Female Cynomolgus. Methods Ten 16 month old female cynomolgus were randomly assigned to exposure and control groups, each group has 5 animals. The exposure group was given50mg 4-Nonylphenol and 200mg Bisphenol A via oral gavage. Control group was given 5ml of corn oil via oral gavage. Animals were respective dosed before breakfast once daily for 4 weeksThe body weight was recorded once a week. When the study was completed, the serum estrogen level and calculate the maturation index of vagina exfoliative cell was tested. The uterus was collected and the wet weight, endometrial and uterine smooth muscle thickness, endometrial epithelial and glandular epithelium height was measured. Results Compared to control group, the serum estrogen level and maturation index of vagina exfoliative cell were significantly higher in exposure group (P<0.05). The uterus wet weight, endometrial and uterine smooth muscle thickness, endometrial epithelial and glandular epithelium height also significantly increased in exposure group (P<0.01). Conclusion The Bisphenol A and 4-Nonylphenol have some reproductive endocrine toxic effects to juvenile female cynomolgus.

Key words: Bisphenol A, 4-Nonylphenol, Juvenile female cynomolgus, Estrogen-like activity