Dynamic Observation On Growth of Subcutaneous and Orthotopic Gastric Tumor by in vivo Fluorescence Imager

doi:10.3969/j.issn.1674-5817.2011.05.015

Abstract

Abstract: Objective To observe the growth profile of MKN-45-Luc gastric tumor in nude mice by fluorescence imaging system of small animals in vivo , after the tumor transplantation into the nude mice through subcutaneous or orthotopic transplantation. Methods To inoculate subcutaneously the MKN-45 gastric cancer cells which were transfected Luc into nude mice, when the tumor formed, it were harvested and inoculated subcutaneously or directly transplanted into the stomach of nude mice. AT the same time, we used the fluorescence imaging system of small animals in vivo to monitor the dynamic changes of MKN-45-Luc cells in mice. Results From the first week to the fifth week, both the luciferase expressed area of the subcutaneous and orthotopic tumor and the fluorescent intensity were gradually increased with the time increase of tumor transplantation . A positive correlation existed among the tumor volume, fluorescence area and fluorescent intensity (r=0.882, P<0.001), which disappeared after 5-6 weeks following the inoculation. Conclusions The fluorescence imaging systems of small animals in vivo were untilized to fully observe the growth and metastasis profile of gastric cancer, which provided the basis for investigating the biological characteristics of the MKN-45 gastric cancer.

Key words: Fluorescence imaging, Subcutaneous and orthotopic transplantation, Gastric cancer, Luciferase

CLC Number:

Q95-33

PENG Xiu-Hua, SHEN Yan, XU Chun-Hua, YANG Yu-Qin, ZHOU Wen-Jiang. Dynamic Observation On Growth of Subcutaneous and Orthotopic Gastric Tumor by in vivo Fluorescence Imager[J]. Laboratory Animal and Comparative Medicine, 2011, 31(5): 371-375.

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