The Comparison of Genetic Polymorphism of SLA-DQA Gene Between Diannan Small-Ear Pig and Yunnan Wild Boar

doi:10.3969/j.issn.1674-5817.2011.05.012

Laboratory Animal and Comparative Medicine ›› 2011, Vol. 31 ›› Issue (5): 360-364.DOI: 10.3969/j.issn.1674-5817.2011.05.012

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The Comparison of Genetic Polymorphism of SLA-DQA Gene Between Diannan Small-Ear Pig and Yunnan Wild Boar

LIU Rong-hui^1,2,3, LU Li-yi¹, TAO Xuan^1,2, YU Hui¹, LI Hua¹

1. Department of Animal Science, Foshan University, Nanhai 528231, China;
2. College of Animal Science and Technology of Sichuan Agriculture University, Ya’an 625014,China;
3. The Kaixian Bureau of Husbandry and Veteriary, Chongqing 400000,China

Received:2011-05-30 Online:2011-10-15 Published:2011-10-25

Abstract

Abstract: Objective Analyzed genotype and allelic polymorphism of SLA-DQA gene in Diannan small-ear pig and Yunnan wild boar (YWB, S. s. silvianus) to detect whether the genotype frequency of restriction enzyme sites was fit with Hardy-Weinberg equilibrium. Methods PCR-RFLP was used to analyze the partial sequences of intron 1 and intron 2 and the complete sequence of extron 2 of SLA-DQA in Diannan small-ear pig(DN) and Yunnan wild boar. Result Both EcoRⅠand AluⅠdigested sites had three kinds of genotypes, There were nine composite genotypes in Banna pig(BN) and eight in Mingguang pig(MG), but only four composite genotypes in Yunnan wild boar. The χ² test value of genotypes at both EcoRⅠand AluⅠsites in Diannan small-ear pig wasn’t fit with Hardy-Weinberg equilibrium (P<0.05), versus in Mingguang pig and Yunnan wild boar was fit with Hardy-Weinberg equilibrium, respectively (P>0.05). Conclusion Genetic polymorphism of SLA-DQA at both EcoR˘ńand AluⅠsites in Diannan small-ear pig is moderate, at EcoRⅠsite in Yunnan wild boar is moderate versus at AluⅠsite in Yunnan wild boar is low; the disequilibrium of Hardy-Weinberg in Diannan small-ear pig is majorly resulted from Banna pig.

Key words: Diannan small-ear pig, Yunnan wild boar, SLA-DQA, RFLP, Hereditary character

CLC Number:

Q95-33

LIU Rong-hui, LU Li-yi, TAO Xuan, YU Hui, LI Hua. The Comparison of Genetic Polymorphism of SLA-DQA Gene Between Diannan Small-Ear Pig and Yunnan Wild Boar[J]. Laboratory Animal and Comparative Medicine, 2011, 31(5): 360-364.

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The Comparison of Genetic Polymorphism of SLA-DQA Gene Between Diannan Small-Ear Pig and Yunnan Wild Boar

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