Abstract: Objective To find out the application value of refinement in consumer health food .Methods Based on the analysis result of contents of the tested samples, eight parameters including fatigue relief and increasing immune function were accessed by adopting “refinement” methods, in accordance with general procedure and specifically designated principle. Results (1) The data about fatigue relief and increasing immune function were collected by the study carried on 120 mice which is less than common using. (2) Those health food show the good effect on fatigue relief and increasing immune in primary study. Conclusion The refinement method combined with designed requirement method could obtain necessary information with less numbers of laboratory animals.

Key words: Refinement, Health food, Functionality experiment