Abstract: Objective Improve the method of making model and macro-evaluation about Premenstrual Syndrome Liver-qi Invasion, Depression Rats Model, providing a “zheng” animal model for the research of mechanism on emotional disease. Methods Adopted ethology and improved vaginal smear to determine rats oestrous cycle，apply open-field method to filt similar scoring rats entering experiment, adjust stimulus intensity and persistence time, introduce open-field method to estimate rats macroscopic appearance. Results Liver-qi invasion rats，scores were much higher than the control while liver-qi depression rats，scores decreasing notably, the trend was retrieved after administration. Conclusion The rat model were consistent with clinical manifestation and pathological changes satisfactorily. Modeling method was reliable and mature with a better credibility and repeatability. Adopting open-field method could distinguish syndrome belongings of rats model clearly. Thus provided a direct evidence for differentiation of symptoms and signs on animal models.

Key words: Premenstrual Syndrome, Liver-qi Invasion, Liver-qi Depression, Animal Model, Open-field