Abstract: Objective To study the effects of hVEGF on the expression of Fn and integrinjA5 during the healing process of rats，full-thickness cutaneous defect which covered by genetically membrane planted by fibroblast integrated with hVEGF recombinant. Methods Wounds with full-thickness cutaneous defect were produced on the dorsum of the SD rats. The rats were randomly divided into four groups. For the rats in the experimented group，the wounds were covered with hVEGF genetically engineering membrane. For the three control groups，the wounds were covered with membrane planted by the fibroblast integrated with blank plasmid，blank membrane and Tegaderm membrane respectively. Specimens were obtained at the 3,7,14 and 29 days after injury, immunohistochemistry was employed to detect the changes in Fn and integrina5 expression, image analysis was used to determine changes of these expression level by detecting the mean optical density value of their expression. Results The expression level of Fn in experimental group was significant higher than that in control groups at 3, 7 and 14d after injury, by detecting the mean optical density value of its expression (P＜0.05). At 14 and 29d after injury, the expression level of Integrina5 in experimental group was significant higher than that in control groups (P＜0.05). Conclusion The application of the hVEGF genetically engineering membrane on the full-thickness cutaneous defect upregulated the expression of Fn in experimental group, especially in the early and metaphase of wound healing. Integrina5 expression significantly increased at metaphase and later period of wound healing. These could accelerate the healing process.

Key words: Human vascular endothelial growth factor, Fn, Integrina5, Wound healing