Abstract: Objective To investigate the changes of TNF-a, IL-10, glucocorticoid receptor (GR) in Rats with acute necrotizing pancreatitis (ANP) and the influence of Dexamethasone(DEX) on these parameters in ANP rats. Methods Fourty-eight male Sprague-Dawley (SD) rats were randomly divided into acute-necrotizing-pancreatitis group (group A,n=16)，DEX-treated group (group D, n=16) and sham-operation group(group S，n=16). ANP model was induced by intergraded injection of 5% sodium tanrocholate to biliopancreatic duct in the rats except for group S, and the rats in group D were intramuscularly given dexamethasone after operation. The change of pancreatic pathology, the serum AMY, TNF-a and IL-10 level, and the blood mRNA expression of GR were examined. Results ①Typical ANP pathological changes were found in group A, including i.e. interstitial edema, infiltration of inflammatory cells, hemorrhage and parenchyma necrosis; and the mild inflammation was found in rats of D group which treated by Dexamethasone.② The concentration of serum TNF-a, IL-10 in group A were obviously higher than that in group S (P＜0.01). The concentration of serum TKF-a decreasing and IL-10 increasing in rats were induced by the usage of Dexamethasone (P＜0.01).③ The mRNA expression of blood GR in group D was lower significantly at 8h or 12h after the operation than that in group S (P＜0.01 or P＜0.05). Conclusions The changes of TKF-a, IL-10, GR are involved in the inflammation response of rat ANP, and the dexamethasone can relieve ANP inflammation response by affecting these factors.

Key words: Dexamethasone, Acute necrotizing pancreatitis, TKF-α, IL-10, Glucocorticoid receptor