Abstract: Ca²⁺currents were obtained in acutely dissociated mouse spermatogenic cells using whole-cell patch clamp technique. The results indicated that inward currents were recorded when K⁺ currents were blocked by Cs⁺and TEA⁺stepping membrane potential to voltage between —80 and +10 mV，in 10 mV increments from a holding potential of —90 mV，the peak amplitude occurred at between —30 mV and —40 mV，and the inward currents remained unchanged in the presence of 4 μmol/L tetrodoxin （TTX）,a special sodium channels blocker，suggesting that it was generated by opening Ca²⁺channels other than Na⁺channels.

Key words: Spermatocyte, Whole-cell patch clamp technique, Ca²⁺channels