Abstract: Biotin carboxyl anti - antibody and enzyme - labled streptoavidin were used in the indirect enzyme - linked immunosorbent assay (ELISA) for the detection of antibodies to mouse hepatitis viruses (MHV) in mice. The results showed that it,s sensitivity was four times higher than that of the ELISA with enzyme -labled staphylococcal protein A, and it's specificity was also better than the latter.

Key words: Biotin-streptoavidin system, Mouse hepatitis viruse, Enzyme-linked immunosorbent assay