Study on in Vivo Survival and Differentiation of Human Umbilical Cord Mesenchymal Stem Cells in Fetal Goat Liver

doi:10.3969/j.issn.1674-5817.2019.05.005

Abstract

Abstract: Objective To observe the survival and differentiation of human umbilical cord mesenchymal stem cells (hUCMSC) in fetal goat liver. Methods Liver tissues of 1 month old lambs was harvest which was injected with GFP-hUCMSC into liver at 2 month fetal phase, and the distribution of green fluorescent protein (GFP)-positive cells were observed under fluorescence microscope. The presence of human sex-Determining Region Y (SRY) gene was detected by PCR, while the expression of human hepatocyte-specific genes were detected by RT-PCR, and the expression of human hepatocyte-specific proteins were detected by immunohistochemical staining. The same-month-old lambs injected with the same volume physiological saline during fetal phase as control. Results The regular or scattered distribution of green fluorescence and the presence of human SRY gene were observed in the liver tissue of 1 month old lambs injected with GFP-hUCMSC. The human hepatocyte nuclear factor 4α(HNF4α), HNF3β and albumin (ALB) gene were positive detected by RT-PCR, and the ALB and HNF4α proteins were positive detected by immunohistochemical staining. No green fluorescence, human SRY genes, human hepatocyte specific genes and proteins were detected in the control lamb liver. Conclusion hUCMSC can survive and differentiate into human hepatocyte-like cells in fetal sheep liver, which can last for at least one month after birth.

Key words: Human umbilical cord mesenchymal stem cells (hUCMSC), Goat, Hepatocyte, Differentiation

CLC Number:

Q95-33

YAN Dong-hai, HUANG Xiao-kun, XIANG Xue-ping, QING De-ke, FANG Wei, LI Zi-an, WANG Qiang, WANG Jin-xiang, PANG Rong-qing. Study on in Vivo Survival and Differentiation of Human Umbilical Cord Mesenchymal Stem Cells in Fetal Goat Liver[J]. Laboratory Animal and Comparative Medicine, 2019, 39(5): 364-370.

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