Abstract: Objective To validate high throughput sequencing results by fluorescence quantitative PCR technique and analyze the expression of miR-200b-3p and miR-200b-5p in bursa, and explore possible molecular mechanism associated with Marek’s disease (MD) resistance/susceptibility. Methods Through optimization, the ideal amplification conditions and reaction system of real-time fluorescence quantitative PCR were identified. Results miR-200b-3p and miR-200b-5p fluorescence quantitative PCR results and high throughput sequencing results were consistent in down-regulation, but not significant difference. miR-200b-3p and miR-200b-5p two homologous miRNA showed similar expression after infected by Marek’s disease virus (MDV). Conclusion Although the difference is not consistent with the results of significant analysis, miR-200b-3p and miR-200b-5p may be associated with resistance / susceptibility of MD.

Key words: MiR-200b, Marek's disease (MD), Resistance/susceptibility, High throughput sequencing, Fluorescence quantitative PCR