Abstract: Objective Through in vitro mesangial cell culture experiments, to explore if the total flavonoids of Qiji Shenkang Granule is the key effective component in the Fufang, and to further explore its mechanism. Method Used the macroporous resin adsorption method to extract total flavonoids in Fufang, and then use vascular tension angiotensin II to induce apoptosis in cultured mesangial cell membrane, which increased the extracellular matrix, after the intervention of total flavonoids of Qiji Shenkang Granule, analyzed the effect of interleukin 6 (IL-6) and monocyte chemotaxis protein-1 (MCP-1), fibronectin (FN) and type IV collagen (ColIV) protein expression. Results the Qiji Shenkang Granule flavonoids in high, dose group significantly reduced IL-6 and MCP-1 protein expression, FN and collagen content were also reduced, compared with Ang II group, P<0.01. Conclusion By regulating the expression of pro-inflammatory factors, the total flavonoids in the Qi Ji Shen Kang Granule can inhibit the excessive accumulation of extracellular matrix.

Key words: Qiji Shenkang Granule total flavonoids, Interleukin 6(IL-6), Monocyte chemotaxis protein-1 (MCP-1), Fibronectin (FN), Type IV collagen (ColIV)