Abstract: Objective To approach feasible of autoimmune myocarditis induced by immunization of mice with bovine serum albumin, to proved a new animal model of autoimmune myocarditis. Methods Experimental group and control group were subcutaneously treated with 0.5 ml 10% bovine serum albumin and physiological saline respectively on day 0, 3, 5, and 10, and then related tests were conducted on day 14 and 28 to confirm if the model was established successfully. Results On day 14 after the first immunization with bovine serum albumin, all BALB/c mice from the experimental group were developed with myocardial interstitial lymphocytic infiltrates, focal myocardial necrosis and haemorrhages, accompanying with endocarditis and epicarditis. Under the electron microscope: It was found myocardial cell and mitochondria swelling, muscle fibers dissolved and lymphocyte infiltration. A high titer of myocardium creatase and more lymphocyte were detected in the immunized mice. On day 28 after the first immunization with bovine serum albumin, the inflammation and myocardial necrosis were not seriously, a same titer of myocardium creatase and less lymphocyte were detected in the immunized mice. Conclusion Autoinmune myocarditis can be induced by immunization of mice with bovine serum albumin, pathological changes of the animal model arre typical, the rate of colony is high.

Key words: Bovine Serum Albumin, Autoimmune, Myocarditis