Effects of Cold Environment on Liver Injury in Dogs after Abdominal Blast Injury

doi:10.12300/j.issn.1674-5817.2025.190

Abstract

Abstract:

[Objective] To investigate the effects of cold environment on physiological parameters, arterial blood gas, liver function, and pathological changes of liver tissue in dogs after abdominal blast injury. [Methods] Ten conventional-grade Beagle dogs were randomly allocated to either a ambient temperature group or a cold exposure group. Both groups underwent abdominal blast injury modeling using a multifunctional animal modeling platform to induce liver injury. Following model establishment, dogs in the ambient temperature group were housed under ambient temperature conditions, while those in the cold exposure group were placed in a cold chamber facility maintained at -25℃. Vital signs, arterial blood gas, and liver function parameters were collected at 24 h before blast injury, immediately after blast injury, and 40 min after blast injury, respectively. Pathological analysis of liver tissues was subsequently conducted. [Results] At 24 h before blast injury and immediately after blast injury, no statistically significant differences were observed in physiological indicators, arterial blood gas, or liver function parameters between the two groups (P>0.05). At 40 min after blast injury, heart rate, body temperature, and systolic blood pressure (SBP) in the cold exposure group differed significantly from those in the ambient temperature group (P<0.05). Compared with the ambient temperature group, the cold exposure group showed a significant increase in partial pressure of carbon dioxide (PCO₂), a marked elevation in the negative value of base excess (BE), and significantly higher levels of total serum bilirubin (TSB), indirect bilirubin (IBIL), alanine aminotransferase (ALT), and aspartate aminotransferase (AST) (P<0.05). Hematoxylin-eosin (HE) staining of liver tissues demonstrated that the cold exposure group presented more severe intrahepatic hemorrhage, more prominent tissue edema, and a more pronounced inflammatory response.. [Conclusion] The cold environment will exacerbate the potential trend of metabolic acidosis after abdominal blast injury and intensify liver damage. In the treatment of trauma in cold environments, it is necessary to strengthen body temperature management, conduct early intervention for acid - base balance, and monitor liver function to reduce the risk of injury.

Key words: Cold environment, Abdominal blast injury, Liver injury, Beagle dogs

CLC Number:

Q95-33

WANG Xinpei,GONG Tiantian,GUO Tiange,et al. Effects of Cold Environment on Liver Injury in Dogs after Abdominal Blast Injury[J]. Laboratory Animal and Comparative Medicine. DOI: 10.12300/j.issn.1674-5817.2025.190.

References 15

[1]	EPSHTEIN E, SHRAGA S, RADOMISLENSKY I, et al. Blast injury and chronic psychiatric disability in military personnel: Exploring the association beyond posttraumatic stress disorder[J]. J Psychiatr Res, 2025, 184: 515-521. DOI: 10.1016/j.jpsychires.2025.03.026 .
[2]	NGUYEN T N, TSUKADA H, JAMES G R, et al. Experimental model and simulant for studying blast penetrating injury to the skin[J]. J Mech Behav Biomed Mater, 2025, 165: 106936. DOI: 10.1016/j.jmbbm.2025.106936 .
[3]	DU W Q, JIANG R Q, ZONG Z W, et al. Establishment of a combat damage control surgery training platform for explosive combined thoraco-abdominal injuries[J]. Chin J Traumatol, 2022, 25(4): 193-200. DOI: 10.1016/j.cjtee.2022.03.003 .
[4]	NGUYEN P D, NAHMIAS J, ARYAN N, et al. Main versus segmental hepatic artery angioembolization in patients with traumatic liver injuries: a Western Trauma Association multicenter study[J]. Surgery, 2025, 178: 108909. DOI: 10.1016/j.surg.2024.10.002 .
[5]	KAZEZIAN Z, BULL A M J. A review of the biomarkers and in vivo models for the diagnosis and treatment of heterotopic ossification following blast and trauma-induced injuries[J]. Bone, 2021, 143: 115765. DOI: 10.1016/j.bone.2020.115765 .
[6]	王家宾, 李青, 刘冬霞, 等. 低温环境下创伤救治决策分析[J]. 创伤外科杂志, 2024, 26(2): 86-90. DOI: 10.3969/j.issn.1009-4237.2024.02.002 .
	WANG J B, LI Q, LIU D X, et al. Decision analysis of trauma care in a low-temperature environment[J]. J Trauma Surg, 2024, 26(2): 86-90. DOI: 10.3969/j.issn.1009-4237.2024.02.002 .
[7]	RUTTER B, SONG H L, DEPALMA R G, et al. Shock wave physics as related to primary non-impact blast-induced traumatic brain injury[J]. Mil Med, 2021, 186(): 601-609. DOI: 10.1093/milmed/usaa290 .
[8]	DENNY J W, DICKINSON A S, LANGDON G S. Defining blast loading 'zones of relevance' for primary blast injury research: a consensus of injury criteria for idealised explosive scenarios[J]. Med Eng Phys, 2021, 93: 83-92. DOI: 10.1016/j.medengphy.2021.05.014 .
[9]	WANG X, DU J, ZHUANG Z, et al. Incidence, casualties and risk characteristics of civilian explosion blast injury in China: 2000—2017 data from the state Administration of Work Safety[J]. Military Med Res, 2020, 7: 29. DOI: 10.1186/s40779-020-00257-5 .
[10]	MACHAKU D, CHAMBO M, NKORONKO M, et al. Ocular, scrotal and abdominal trauma in a secondary blast injury[J]. J Surg Case Rep, 2023, 2023(3): rjad126. DOI: 10.1093/jscr/rjad126 .
[11]	ZHANG W W, SHEN M H, CHU P, et al. Molecular characterization of CIRBP from Takifugu fasciatus and its potential roles in cold-induced liver damage[J]. Int J Biol Macromol, 2024, 281(Pt 2): 136492. DOI: 10.1016/j.ijbiomac.2024.136492 .
[12]	JI Y, WANG Y, FEI P P, et al. Risk factors contributing to inadvertent postoperative hypothermia in adult patients following hepatobiliary surgery[J]. J PeriAnesthesia Nurs, 2026, 41(1): 185-190. DOI: 10.1016/j.jopan.2025.06.005 .
[13]	LIU Q, WANG S Y, FU J, et al. Liver regeneration after injury: Mechanisms, cellular interactions and therapeutic innovations[J]. Clin Transl Med, 2024, 14(8): e1812. DOI: 10.1002/ctm2.1812 .
[14]	TIPISOVA E V, DERNOVOY B F, PROSHEVA V I, et al. Seasonal indicators of catecholamines, acetylcholine, thyroid hormones levels, and functional parameters of the cardiovascular system in males in the European north of Russia[J]. Hum Physiol, 2025, 51(2): 194-200. DOI: 10.1134/S0362119725700318 .
[15]	DUMONT L, RICHARD G, ESPAGNET R, et al. Shivering, but not adipose tissue thermogenesis, increases as a function of mean skin temperature in cold-exposed men and women[J]. Cell Metab, 2025, 37(9): 1789-1805.e4. DOI: 10.1016/j.cmet.2025.06.010 .