Abstract: Objective To prepare anti-IgG of Tupaia belangeri, Cricetulus migratorius and Merionesun guiculatus, and label them by using FITC and HRP. Method The IgG of these three animals were purified using Protein G and the anti-IgG were obtained from immunized rabbits. The anti-IgG were purified through affinity chromatography and labeled by FITC and HRP. Results The anti-IgG concentration of Cricetulus migratorius, Tupaia belangeri and Merionesun guiculatus was 2.2 mg/ml, 2.3 mg/ml and 2.3 mg/ml respectively. The IgG content of FITC labeled anti-IgG for each animal was 1.19 mg/ml, 1.36 mg/ml and 1.55 mg/ml respectively. The IgG content of HRP labeled anti-IgG for each animal was 0.84 mg/ml, 1.09 mg/ml and 1.31mg/ml respectively. Conclusion The study successfully obtained the FITC and HRP labeled anti-IgG, which can be used for the establishment of microorganism detection in these three animals.

Key words: Tupaia belangeri, Cricetulus migratorius, Merionesun guiculatus, IgG, Label