Preliminary Research on Mouse Model of Influenza Virus by Aerosol Infection

doi:10.3969/j.issn.1674-5817.2013.03.003

Abstract

Abstract: Objective Preliminary study on the model of influenza which was established by simulation of human natural infection in mouse. Methods ICR mice were infected with H1N1 FM1 and H1N1 PR8 virus by aerosol inhalation. The symptoms and body weight of mice were observed everyday. At 5, 12, 21 day after infection,the mice were sacrificed. The lungs of mice were weighed, then were done virus assay and pathological observation. Results The clinical symptoms of infection mice were obvious, the body weight lost seriously, lung index increased and pathological changes significantly in the early mid-term, for example the typical pulmonary edema, pulmonary interstitial disease, inflammation and pulmonary congestion appeared in the lung of mice. The influenza virus also can be detected in the early stage. The symptoms of mice getting better, the virus were not detected in the late. Conclusions The established infection mouse model of the influenza virus aerosol by aerosol inhalation is feasible.

Key words: Aerosol, Influenza virus, Mouse, Model

CLC Number:

R33Q95-33

YANG Yu-qin, ZHU Zhao-qin, XU Chun-hua, HE Jing, PENG Xiu-hua, ZHOU Xiao-hui, HU Yun-wen, ZHOU Wen-jiang. Preliminary Research on Mouse Model of Influenza Virus by Aerosol Infection[J]. Laboratory Animal and Comparative Medicine, 2013, 33(3): 180-184.

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