Effect of Latrunculin A on Motility of Spermatozoa Cryopreservation in ICR Mouse

doi:10.3969/j.issn.1674-5817.2013.01.002

Abstract

Abstract: Objective To evaluate the effect of latrunculin A on motility of spermatozoa combined with optimized cooling rate of cryopreservation. Methods ICR mouse epididymal spermatozoa balanced in freezing medium were frozen at fast (-240℃/min), medium (-109℃/min), slow (-41℃/min) and super slow (-12℃/min) cooling rate for 5 min or 10 min respectively, before being plunged into liquid nitrogen for storage. Results No difference of sperm motility was observed between spermatozoa frozen for 5 and 10 min above liquid nitrogen at each cooling rate. Spermatozoa cryopreserved at super slow and medium cooling rate showed highest and lowest post-thaw motility respectively. ICR mouse spermatozoa were treated with 0.5 µmol/L, 1 µmol/L, 2 µmol/L and 4 µmol/L Latrunculin A before freezing and then cryopreserved at super slow cooling rate. The result showed that treating mouse spermatozoa with 0.5 µmol/L, 1 µmol/L and 2 µmol/L of Latrunculin A could not improve the motility of spermatozoa. However, high level (4 µmol/L) of Latrunculin A showed detrimental effect on ICR mouse sperm motility. Conclusion Cryopreservation proficiency of Latrunculin A on sperm is open to discussion.

Key words: Mouse spermatozoa, Cooling rate, Latrunculin A, Motility

CLC Number:

Q95-33

CHENG Fu-rong, YANG Shi-hua. Effect of Latrunculin A on Motility of Spermatozoa Cryopreservation in ICR Mouse[J]. Laboratory Animal and Comparative Medicine, 2013, 33(1): 7-11.

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