Laboratory Animal and Comparative Medicine ›› 2015, Vol. 35 ›› Issue (5): 383-389.DOI: 10.3969/j.issn.1674-5817.2015.05.008

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The Forelimb Bone Mineral Density and Expression of Calcitonin and Osteocalcin in Forelimb Malformed White Hair & Black Eyes Rabbits

CHEN Fang-ming, LIN Lin, CAI Yue-qin, CHEN Cheng, CHEN Min-li   

  1. The Research Center of Animal Experiment, Zhejiang Chinese Medicine University, Hangzhou 310053, China
  • Received:2014-12-11 Online:2015-10-25 Published:2015-10-25

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Abstract: Objective To observe bone mineral density and expression level of calcitonin (CT), osteocalcin (also named bone gla protein, BGP) proteins in forelimb malformation WHBE rabbits (FMWHBE) and to explore possible cause of forelimb malformation and possibility to be an animal model for bone biomechanical studies. Method FMWHBE rabbits, WHBE rabbits and Japan white rabbits(JW) at 1, 2, 3 and 4 months of age were selected with six rabbits for each age. Contents of CT, BGP in serum were detected after body weighing and a small animal imaging system was used to measuring bone mineral density of proximal end, middle end and telecentric end in forelimb humerus, radius and ulna. CT and BGP immunohistochemical staining were used for pathological observation after decalcification. Result No significant difference of body weights of each month old was observed among all groups. Bone mineral density of whole length in 1 month old and proximal end, middle end in 2 months old and telecentric end in 3 months old of humerus, middle end and telecentric end in 1 month and telecentric end in 2 and 3 months old of radius and whole length in 1 and 2 months old and telecentric end in 3 months old of ulna in FMWHBE rabbits were notably lower than that in WHBE and JW rabbits (P<0.05 or P<0.01). CT and BGP contents in serum of 1 month old FMWHBE were notably decreased (P<0.05 or P<0.01). BGP level in 2 months old was obviously higher than that of in JW rabbits(P<0.01). CT level in 3 month olds was notably higher than that in WHBE rabbits (P<0.05). Immunohistochemical staining of CT, BGP showed that positive parts of CT expression in FMWHBE rabbit was less and weaker, and expression of BGP in FMWHBE rabbits was slightly weaker than that in JW rabbits and WHBE rabbits. Conclusion Forelimb malformation formation and bone mineral density changes in FMWHBE rabbits may be related with the special stress in forelimb bones and expression character of CT and BGP induced by lying posture during growth.

Key words: Forelimb malformed, WHBE rabbits, Bone mineral density, Calcitonin (CT), Osteocalcin (BGP)

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