Generation and Genotyping of Monocyte-Peroxisome Proliferator-activated Receptor γ Conditional Knockout Mice

doi:10.3969/j.issn.1674-5817.2018.03.011

Laboratory Animal and Comparative Medicine ›› 2018, Vol. 38 ›› Issue (3): 222-226.DOI: 10.3969/j.issn.1674-5817.2018.03.011

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Generation and Genotyping of Monocyte-Peroxisome Proliferator-activated Receptor γ Conditional Knockout Mice

ZHENG Xin-xun¹, ZHANG Zhi-jing¹, HUANG Bao-yi¹, LIAO Zi-jun², LIU Jian-jun³, LUO Tao¹

1. Peking University Shenzhen Hospital,Shenzhen 518035,China;
2. The Second People's Hospital of Shenzhen,Shenzhen 518035,China;
3. Shenzhen Center for Disease Control and Prevention,Shenzhen 518000,China

Received:2017-07-07 Revised:2018-04-22 Online:2018-06-25 Published:2021-03-01

Abstract

Abstract: Objective To generate monocyte- peroxisome proliferator-activated receptor γ(PPARγ) conditional knockout mice and to identify the genotype.Methods The imported PPARγ-loxp mice and CX3CR1-Cre mice were hybridized to generate F1 generation,and the genotypes were identified by RT-PCR.Using the same method to generate F2 generation and detect their genotypes.Then the effect of PPARγgene knock-out were identified by using immunofluorescence and Western blotting.Result The monocyte-PPARγknockout mice (PPARγ^loxp/loxp Cx3cr1^cre/- or PPARγ^loxp/loxp Cx3cr1^cre/cre) were as test group(PPARγko),and the control group(PPARγwt) of mice (PPARγ^loxp/loxp Cx3cr1^-/-) were also identified.Conclusion Successfully established the monocyte-PPARγknock-out mice,and provide a experimental tool to further study the funtions of PPARγon monocyte .

Key words: Peroxisome proliferator-activated receptor γ(PPARγ), Conditional knockout, Cre-loxp System, Monocyte.

CLC Number:

Q95-33

ZHENG Xin-xun, ZHANG Zhi-jing, HUANG Bao-yi, LIAO Zi-jun, LIU Jian-jun, LUO Tao. Generation and Genotyping of Monocyte-Peroxisome Proliferator-activated Receptor γ Conditional Knockout Mice[J]. Laboratory Animal and Comparative Medicine, 2018, 38(3): 222-226.

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Generation and Genotyping of Monocyte-Peroxisome Proliferator-activated Receptor γ Conditional Knockout Mice

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