Using Several Alternative Test Methods Strategically Combination to Assess Eye Irritation/Corrosion of 1,2-hexanediol

doi:10.3969/j.issn.1674-5817.2018.03.010

Laboratory Animal and Comparative Medicine ›› 2018, Vol. 38 ›› Issue (3): 217-221.DOI: 10.3969/j.issn.1674-5817.2018.03.010

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Using Several Alternative Test Methods Strategically Combination to Assess Eye Irritation/Corrosion of 1,2-hexanediol

CHEN Xiu-juan, HUANG Yu-feng, LIU Xiang-mei, PANG Zeng-xiong, JIANG-Yi, LI Pei-ning, LI Guang-xian, CHEN Zi-ling, SUN Xia, WANG Qiang

Guangzhou Quality Supervision and Testing Institute,Guangzhou 511447,China

Received:2018-03-09 Online:2018-06-25 Published:2021-03-01

Abstract

Abstract: Objective Using several alternative test methods strategically combination to assess eye irritation/corrosion of different concentrations of 1,2-hexanediol.Methods With the bottom-up approach,two different sensitive degree of tests,the hen’s egg test - chorioallantoic membrane (HET-CAM) and the bovine corneal opacity and permeability (BCOP) in vitro were adapted to test eye irritation of 1,2-hexanediol in different concentrations,and finally the safe range of eye irritation was confirmed by Draize eye test.Results HET-CAM test:1,2-hexanediol was non-irritant when the concentration was below volume fraction 0.625% and light irritant at volume fraction 1.25% concentration,moderate irritation at volume fraction 2.50% concentration.In the concentration of volume fraction 5.00% and 10.00%,1,2-hexanediol induced serious eye damage.BCOP test:1,2-hexanediol at volume fraction 2.50% and 5.00% concentration induced light irritation or irritation,and severe corrosive in the concentration of volume fraction 10.00%.Confirmed by the Draize test,1,2-hexanediol was non-irritant at volume fraction 5.00% concentration,light irritation at volume fraction 10.00% concentration.Conclusion In this condition,1,2-hexanediol was non-irritant with the concentration lower than volume fraction 5.00%.

Key words: 1,2-hexanediol, Ocular irritation, Alternative method, Bovine corneal opacity and permeability (BCOP), Hen‘s egg test - chorioallantoic membrane (HET-CAM), Rabbit eye irritant test (Draize test)

CLC Number:

Q95-33

CHEN Xiu-juan, HUANG Yu-feng, LIU Xiang-mei, PANG Zeng-xiong, JIANG-Yi, LI Pei-ning, LI Guang-xian, CHEN Zi-ling, SUN Xia, WANG Qiang. Using Several Alternative Test Methods Strategically Combination to Assess Eye Irritation/Corrosion of 1,2-hexanediol[J]. Laboratory Animal and Comparative Medicine, 2018, 38(3): 217-221.

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Using Several Alternative Test Methods Strategically Combination to Assess Eye Irritation/Corrosion of 1,2-hexanediol

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