Abstract: Objective To screen metastasis-associated genes by using highly metastatic animal and cell line for the purpose of the biomarker exploration of human lung cancer metastasis. Methods RNA from high metastatic SPC-A-1 sci cells and the low metastatic SPC-A-1 cells were isolated and purified，respectively. Differential profil-ing of gene expression between the two cells were screened by cDNA microarray. Western analyzed the expression of some differentally expressed genes. Results Total of 7649 genes, including 3891 genes up-regulated and 3758 genes down-regulated, were found to differentially express between the high metastatic and low metastatic cells by cDNA microarray. Those genes involved in adhesion, apoptosis, transcription, signal transduction, cell cycle and metabolism. After Western blot analysis, CD44 and CDC42EP3 in the SPC-A-1 sci expression was significantly higher than that of SPC-A-1, while the opposite result in FN1. This is consistent with the results of gene chips.Conclusions More than 7000 differential expressed genes might be associated with metastasis of human lung cancer. This study provided a promising reference for mechanism exploration of lung cancer metastasis and new metastatic related genes discovery.

Key words: Human lung cancer, Metastasis-associated genes, cDNA microarray, Western blot