Laboratory Animal and Comparative Medicine ›› 2008, Vol. 28 ›› Issue (6): 387-389.

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Study on Relationship Between Synaptophysin in Adrenal Glands and Postmortem Interval in Rats

  

  1. 1. Department of Forensic Pathology, China Medical University School of Forensic Medicine, Shenyang 110001, China;2. Liaoning Province Institute of Criminal Science and Technology, Shenyang 110032, China;3. Key Lab of Laboratory Transgenic Animal of Liaoning Province, Department of Laboratory Animal Science,China Medical University, Shenyang 110001, China
  • Received:2008-09-05 Online:2008-06-30 Published:2008-06-30

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Abstract: Objectives To explore the changing of synaptophysin after death, and describe the relationship between the changing of synaptophysin and postmortem interval (PMI). Methods Rats were killed by cervical dislocation. Adrenal glands were taken out at different time after death. The synaptophysin in adrenal glands was detected by immunohistochemistry，and the average gray value of synaptophysin was measured by image analyzing system . The average gray values of different group were analyzed by SPSS. Results Synaptophysin in adrenal glands was stable during the first 12h after death, and then it decreased rapidly from 16h to 24 h after death. Conclusion Degradation of synaptophysin in adrenal glands varies with PMI, synaptophysin might be a new indicator for estimating PMI.

Key words: Synaptophysin, Postmortem interval (PMI), Immunohistochemistry, Forensic pathology