Abstract: Objective To study the therapeutic effect of combining angiostatin gene therapy with peritoneal chemotherapy with 5-FU on cachexia in nude mice bearing with colorectal carcinoma metastasis in abdominal cavity. Methods Angiostatin gene was cloned into adenovirus with method of RT-PCR. LoVo cell suspension was i.p to 40 nude mice. The mice were treated with combining angiostatin gene therapy and 5-FU peritoneal chemotherapy or others. Observation were made on the improvement of physiological conditions of the cachetic nude mice after treatment, and the serum levels of TNF-a and IL-1 were assayed by ELISA method. Result Compared with the controlled groups, the group of combining therapy was less loss of body mass, higher food intake, better mental condition and lower levels of TNF-a and IL-1. Conclusion Combining angiostatin gene therapy and 5-FU peritoneal chemotherapy has beneficial effect on cachexia in nude mice bearing with colorectal carcinoma in abdominal cavity.

Key words: Angiostatin, Colorectal Carcinoma, 5-FU, Gene Therapy, Cachexia