Abstract: Using the three times （interval 4 hours） continual partial hepatectomy （CPH） rat as model,the changes in activity of the acid phosphatase （ACP）, alkaline phosphatase （AKP），and content of constitutive heat shock protein70/induced heat shock protein68 （HSC70/HSP68） and proliferating cell nuclear antigens （PCNA） during rat liver regeneration were analyzed. The results showed that their activities and con-tents were increased 4 hours after the lobus sinister was resected at the first time partial hepatectomy （PH）.The activity of the ACP and content of PCNA were negatively relat-ed with AKP and HSC70/HSP68 4 hours after the 2nd and 3rd time PH respectively,when the centralis and dexter were resected,and that the PCNA expression was positive with the number of sequence of CPH.Based on changes of the distribution，content and activity of ACP and AKP in the rat liver by CPH，it was inferred that the AKP located in cell membrane regulated the cell division and differentiation through activiating ACP in cytoplasm，and that PCNA and HSC70/HSP68 may play an important role in liver regeneration by participating in synthesis,transport，selective degradation of proteins and DNA replication.

Key words: Continual partial hepatectomy, Liver regeneration, Constitutive heat shock protein70/induced heat shock protein68, Acid phosphatase, Alkaline phos-phatase, Proliferating cell nuclear antigens