Abstract: Objective To establish human lung cancer cell line and human liver cancer cell line with GFP/Luc double-tagging which having high expressing rate，and to observe development of the tumors in deep organisms of the nude mice with in vivo biofluorescence imaging. Methods The SPC-A-1-GFP/Luc cell line and SMMC-7721 -GFP/Luc cell line were established by lentivirus transfection, en-hancing their GFP/Luc double-tagging expressing rate to about 100% by flow cytometer, then were inoculated in lung and liver of the BALB/c nude mice respectively, finally, the behaviors of the two cell lines transfected stably in development of the tumours was detected by in vivo biofluorescence imaging.Results The cell lines of SPC-A-1-GFP/Luc and SMMC-7721-GFP/Luc with GFP/Luc double-taking have been built up with the expressing rate nearly 100%. After the two cell lines have been inoculated in lung and liver of the nude mice, the tiny tumor focuses in early time and the status of tumors in late time in the deep organisms were observed by the in vivo biofluorescence imaging. Conclusion The estab-lished cell lines with stable GFP/Luc double-tagging, coupled with the application of m vivo biofluorescence imaging, can detect the tumors in deep organisms of the nude mouse intravitaly，real-timely, quantitively,and may be offering an ideal device for researchers to further explore development of the tumor.

Key words: GFP/Luc double-tagging, Lentivirus carrier, In vivo biofluorescence imaging;Tumour model