Abstract: RNA interference (RNAi)has been extensively used for sequence-specific silencing of target gene. The generation of RNAi transgenic mice made it possible to knock down gene expression in mammalian species. This paper reviewed the generation of transgenic RNAi mice and its application. The contribution of RNAi transgenic mice to functional genomics and of its prospect for application were also discussed.

Key words: Trans gene, RNAi, Intracytoplasmic Sperm Injection