Abstract: Objective To establish melioidosis model of FMMU albino guinea pig. Method FMMU albino guinea pig infection model were made by intraperitoneal inoculation of Burkholderia pseudomallei. The clinical manifestations, the amount of bacteria in blood，mortality rate and histopathologic changes in major parenchymatous organs were observed, the killing effect of polymorphonuclear leukocyte on Burkholderia pseudomallei was observed by electron micro scope (EM). Result The characteristic sign of the melioidosis appeared after inoculation, the amount of bacteria in blood was 1 4000 CFU/ml at 72 h post inoculation，mortality rate was 70% at 96 h post inoculation. Under light microscope there were typical suppuration focus in parenchymatous organ. Polymorphonuclear leukocyte could kill the Burkholderia pseudomallei when observed under EM. Conclusion Burkholderia pseudomallei infection model of FMMU albino guinea pig was established successfully and may be used for research on acute Melioidosis in human.

Key words: Melioidosis, Burkholderia pseudomallei, FMMU albino guinea pig