Abstract: Objective    To assess the influence on C57BL/6J mice and diabetic model db/db, ob/ob mice by feeding with whole plant protein formula diet. Methods    Fifty male and 50 female C57BL/6J mice were divided into normal fish protein-based diet control group and whole plant protein formula diet experiment group,  20 female db/db and 20 female ob/ob mice were randomly divided into normal diabetic model diet group and whole plant protein formula diabetic model diet group, respectively. All the mice were 4 weeks old and the period of experiment was lasted for 16 weeks. During the experiment, the daily situation and every 4 weeks blood glucose changes in each group of mice were observed. After 16 weeks, the urine samples were used for studying metabonomics, the blood samples of each group were collected to test blood biochemistry, and the part of free gossypol target organs like liver, kidney, and pancreas were taken for histological observation. Results    No discernible difference was observed in body weight, blood glucose and histological changes between the experiment group and the control group, respectively. A series of metabonomics studies revealed some differences between the experiment group and the control group in the urine, in which the concentrations of leucine, isoleucine, tyrosine and threonine were different. Conclusion     Through the study of nutriology, toxicology and metabonomics, it is preliminarily believed that the replacement of fish protein by degossypolled cottonseed protein in laboratory animal diet is safe.

Key words: Metabonomics, Whole plant protein formula diet, Degossypolled cottonseed protein,