The Characteristics of Anti-chemical Cancer Inducing Factors in Naked Mole-rat

doi:10.3969/j.issn.1674-5817.2019.06.011

Abstract

Abstract: Objective Chemical carcinogens were used to induce naked mole-rats and mice, respectively, to study the characteristics of anti-chemical carcinogens in naked mole-rats. Methods In the adult naked mole-rats (52 weeks) and the adult mice (8 weeks), urethane and o-aminoazotoluene (OAAT) were respectively used to induce tumor formation in the two groups of animals for 3 months.The incidence of tumor in animals were observed, the leukocyte count and body weight of the animals were detected after urethane injection. The pathological changes in the main organs of the animals were observed after OAAT smear. Results After 3 months of urethane mutagenesis, lung tumors could be observed in the mice, and the average body mass continued to decline and the content of white blood cells significantly decreased. Two months after the end of mutagenesis, the incidence of the tumor was more than 50%. However, the naked mole-rat group still maintained a healthy physiological state, with no significant changes in average body weight, white blood cell indicators. Three months after OAAT mutagenesis, obvious pathological changes occurred in thymus, spleen, liver, small intestine and bone marrow of mice, while no obvious lesions were found in the corresponding organs and tissues of naked mole-rat. Conclusion Naked mole-rats have natural resistance to chemical carcinogens, and their living conditions, biochemical indicators, and the morphology and functions of tissues and organs cannot be affected by external chemical carcinogens.

Key words: Naked mole-rat, Urethane, O-aminoazotoluene (OAAT), Antitumor

CLC Number:

Q95-33

CHEN Chao, CONG Wei, YANG Wen-jing, CUI Shu-fang. The Characteristics of Anti-chemical Cancer Inducing Factors in Naked Mole-rat[J]. Laboratory Animal and Comparative Medicine, 2019, 39(6): 484-488.

References

[1] Buffenstein R.The naked mole-rat: a new long-living model for human aging research[J]. J Gerontol A Biol Sci Med Sci, 2005, 60(11):1369-1377.
[2] Tian X, Azpurua J, Hine C, et al.High-molecular-mass hyaluronan mediates the cancer resistance of the naked mole rat[J]. Nature, 2013, 499(7458):346-349.
[3] 杨文静, 崔淑芳. 普通级封闭群裸鼹鼠种群的建立及其生物学特性的研究进展[J]. 中国实验动物学报, 2018, 26(4):518-522.
[4] Zeng YW, Yang JZ, Pu XY, et al.Strategies of functional food for cancer prevention in human beings[J]. Asian Pac J Cancer Prev, 2013, 14(3):1585-1592.
[5] 熊秋迎, 朱金华, 孙昊鑫, 等. 清燥救肺汤对乌拉坦诱导肺癌模型鼠TGF-β1、Smad2和p38 MAPK的影响[J]. 中药药理与临床, 2018, 34(3):14-18.
[6] 徐颖, 肖海龙, 蔡曹盛, 等. 液相色谱-串联质谱法测定化妆品中9种禁用芳香胺[J]. 理化检验:化学分册, 2014, 6(50):715-719.
[7] 王瑞, 徐琳燕, 邱狄, 等. 快速溶剂萃取-凝胶渗透色谱净化-气相色谱质谱法测定土壤中邻甲苯胺含量[J]. 化学分析计量, 2016, 25(5):80-84.
[8] 陈晨, 盛云华, 王宇, 等. 乌拉坦致C57BL/6J 小鼠肺腺瘤动物模型方法研究[J]. 中国药学杂志, 2018, 53(9):701-706.
[9] Gladyshev VN, Zhang G, Wang J.The naked mole-rat genome:understanding aging through genome analysis[J]. Aging(Albany NY), 2011, 3(12):1124-1124.
[10] Seluanov A, Hine C, Azpurua J, et al.Hypersensitivity to contact inhibition provides a clue to cancer resistance of naked mole-rat[J]. Proc Natl Acad Sci USA, 2009, 106(46):19352-19357.
[11] Tan L, Ke Z, Tombline G, et al.Naked mole rat cells have a stable epigenome that resists iPSC reprogramming[J]. Stem Cell Reports, 2017, 9(5):1721-1734.
[12] Yang Z, Zhang Y, Chen L.Single amino acid changes in naked mole rat may reveal new anti-cancer mechanisms in mammals[J]. Gene, 2015, 572(1):101-107.
[13] Seluanov A, Gladyshev VN, Vijg J, et al.Mechanisms of cancer resistance in long-lived mammals[J]. Nat Rev Cancer, 2018, 18(7):433-441.
[14] Peavy DL, Pierce CW.Cell-mediated immune responses in vitro. III. Elimination of specific cytotoxic lymphocyte responses by 3H-thymidine suicide[J]. J Immunol, 1975, 115(6):1521-1524.
[15] Cheng J, Yuan Z, Yang W, et al.Comparative study of macrophages in naked mole rats and ICR mice[J]. Oncotarget, 2017, 8(57):96924-96934.
[16] Majeti R, Chao MP, Alizaden AA, et al.CD47 is an adverse prognostic factor and therapeutic antibody target on human acute myeloid leukemia stem cells[J]. Cell, 2009, 138(2):286-299.
[17] Jaiswal S, Jamieson CH, Pang WW, et al.CD47 is Nupregulated on circulating hematopoietic stem cells and leukemia cells to avoid phagocytosis[J]. Cell, 2009, 138(2):271-285.
[18] Kulkarni A, Chandrasekar V, Natarajan SK, et al.A designer self-assembled supramolecule amplifies macrophage immune responses against aggressive cancer[J]. Nat Biomed Eng, 2018, 2(8):589-599.
[19] Liu M, OConnor RS, Trefely S, et al.Metabolic rewiring of macrophages by CpG potentiates clearance of cancer cells and overcomes tumor-expressed CD47-media-ted'don't-eat-me' signal[J]. Nat Immunol, 2019, 20(3):265-275.