Endoscopy Guided Tracheal Injecting Bleomycin Model of Pulmonary Fibrosis in Mice

doi:10.3969/j.issn.1674-5817.2019.02.005

Abstract

Abstract: Objective To establish intratracheal intubation under direct vision of multifunctional endoscopic, and adopted to establish the model of bleomycin induced pulmonary fibrosis in C57BL/6J mice. Methods Establish method and steps of endotracheal intubation under direct vision of multifunctional endoscopic with five C57BL/6J mice. Ten mice were randomly divided into two groups (5 in each). Control group were given 50 µL of saline and experimental group were given 50 µL of bleomycin (3.5 mg/kg) to observe the death rates of mice. At the 28th day, the mice were sacrificed by cervical delocation. By compared the vitality of superoxide dismutase (SOD), glutathione peroxidase(GSH-PX), the content of malondialdehyde (MDA) and hydroxyproline (HYP) in the two groups of mouse lung tissue. Lung tissue fibrosis degree were observed by HE and Masson stain. Results All C57BL/6J mice were successfully intubated by using multifunctional endoscopy under direct view and no mice died after intubation. The 5 mice given India ink had black materials evenly distributed from trachea bottom until the lungs. Compared with those of control group, HYP and MDA contents in mouse lung tissue experimental group elevated(P<0.01), while SOD and GSH-PX level in mice lung tissue decreased (P<0.01). The lung tissue was obtained and stained by HE and Masson. By comparison with control group, collagen deposition in experimental group mouse lung tissue is serious. The pathological changes were in accordance with pulmonary fibrosis. Conclusion The method of endotracheal intubation under direct vision of multifunctional endoscopic is safe, effective and convenient. This method was successful to establish pulmonary fibrosis in mice model with high efficiency induced by bleomycin, and worthy of popularization and application.

Key words: Endoscopic, Endotracheal intubation, Pulmonary fibrosis, Animal modelsa

CLC Number:

Q95-33

YU Hua-jun, WU Shang, HUANG Hui, LIN Bi-yun, WU Jun, OU Hua-jun, ZHANG Hai-tao. Endoscopy Guided Tracheal Injecting Bleomycin Model of Pulmonary Fibrosis in Mice[J]. Laboratory Animal and Comparative Medicine, 2019, 39(2): 94-98.

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