Establishment of Loop-mediated Isothermal Amplification for Detection of Feline Panleukopenia Virus

doi:10.3969/j.issn.1674-5817.2019.01.007

Laboratory Animal and Comparative Medicine ›› 2019, Vol. 39 ›› Issue (1): 34-38.DOI: 10.3969/j.issn.1674-5817.2019.01.007

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Establishment of Loop-mediated Isothermal Amplification for Detection of Feline Panleukopenia Virus

MA Qin¹, YAN Wen-zhuo¹, ZHOU Jie², GAO Cheng², LIU Tie-long³, ZHAO Li-li¹, CHEN Hong-yan¹, LU Tao-feng¹

1. Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin 100193, China;
2. Shanghai Laboratory Animal Research Center, Shanghai 201203, China;
3. Jiyuan Animal Health Inspection, Jiyuan 454650, China

Received:2018-09-17 Online:2019-02-25 Published:2021-01-29

Abstract

Abstract: Objective To establish a rapid, convenient detection method of feline panleukopenia virus (FPV) by loop-mediated isothermal amplification (LAMP). Methods Two pairs of primers were designed according to the conserved sequences of FPV in Genbank. The efficient LAMP amplification conditions were determined by optimizing temperature and time. Results The results showed a highly efficient amplification for FPV nucleic acid which performed at 65 ℃for 60 min. It also showed a high sensibility with a detection limit of 5.01×102 copies/µL, 10 times higher than the conventional PCR detection in the sensitivity. There was no cross reaction with feline herpesvirus type 1(FHV-1), canine parvovirus(CPV) and vanine adenovirus (CAV). Conclusion The established LAMP method is good in duplication, stability, specificity, and sensitivity. This method can be used for the fast detection of FPV nucleic acid in clinical samples from cats.

Key words: Feline panleukopenia virus(FPV), Loop-mediated isothermal amplification(LAMP), Detecting method

CLC Number:

Q95-33

MA Qin, YAN Wen-zhuo, ZHOU Jie, GAO Cheng, LIU Tie-long, ZHAO Li-li, CHEN Hong-yan, LU Tao-feng. Establishment of Loop-mediated Isothermal Amplification for Detection of Feline Panleukopenia Virus[J]. Laboratory Animal and Comparative Medicine, 2019, 39(1): 34-38.

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Establishment of Loop-mediated Isothermal Amplification for Detection of Feline Panleukopenia Virus

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