Detection of Main Immune Indexes in SCID. Beige and NOD.CB17-Prkdcscid Mice

doi:10.3969/j.issn.1674-5817.2016.05.008

Abstract

Abstract: Objective To measure the immunoglobulin, complement and main immune indexes in SCID. Beige and NOD.SCID mice and analyze their difference. Methods Thirty SCID.Beige and thiry NOD.SCID mice, half male and female, at the age of 4 weeks, 8 weeks and 16 weeks were selected for detecting the main immunoglobulin and complement indexes. Another fourteen SCID.Beige and fourteen NOD.SCID mice, half male and female, at the age of 8 weeks were also selected for detecting the T, B lymphocyte function and NK cell activity. Results The IgG of SCID.Beige at all the age stages were significantly lower than those of NOD.SCID, but the IgA were significantly higher than those of NOD. SCID.The serum C4 of SCID.Beige mice at the age of 4 weeks and 16 weeks were significantly higher than those of NOD.SCID. The IgG of SCID.Beige and NOD.SCID mice at the age of 8 weeks were significantly lower than the other two age stages. In the T cell subsets, the CD4⁺CD8⁺ percentages of SCID.Beige mice at the age of 8 weeks were significantly lower than those of NOD.SCID. Among the same sex and age group of NOD.SCID and SCID.BG, the immune indexes show no obvious differences. Conclusions The antibodies, complement and immune cell levels of SCID.Beige and NOD.SCID are significantly lower than normal mice. They all belong to severe immunodeficiency animals. Among the same age and sex group of the two strains, the immune indexes were different. So the animals should be selected according to the research purpose in the experiments.

Key words: SCID.Beige mice, NOD.CB17-Prkdcscid mice, Immunoglobulin, Complement, Immune cells

CLC Number:

Q95-33

YUAN Shui-juan, SHI Guo-jun, TANG Hui-qing, XU Wen-jing, FU Jie, SHI Mei-lian. Detection of Main Immune Indexes in SCID. Beige and NOD.CB17-Prkdcscid Mice[J]. Laboratory Animal and Comparative Medicine, 2016, 36(5): 365-368.

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